TY - JOUR
T1 - The structure of FemXWv in complex with a peptidyl-RNA conjugate
T2 - mechanism of aminoacyl transfer from Ala-tRNAAla to peptidoglycan precursors
AU - Fonvielle, Matthieu
AU - Li de La Sierra-Gallay, Inés
AU - El-Sagheer, Afaf H.
AU - Lecerf, Maxime
AU - Patin, Delphine
AU - Mellal, Dénia
AU - Mayer, Claudine
AU - Blanot, Didier
AU - Gale, Nittaya
AU - Brown, Tom
AU - van Tilbeurgh, Herman
AU - Ethève-Quelquejeu, Mélanie
AU - Arthur, Michel
PY - 2013/7/8
Y1 - 2013/7/8
N2 - To gain insight into the catalytic mechanism of non-ribosomal amino acid transferases, peptidyl-RNA conjugates were synthesized for co-crystallization with FemXWv of Weissella viridescens, which transfers L-Ala from Ala-tRNAAla to the peptidoglycan precursor UDP-MurNAc-pentapeptide. The structure of the resulting complex and mutational studies revealed the mechanism by which FemXWv binds its substrates for substrate-assisted catalysis and stabilization of the tetrahedral intermediate.
AB - To gain insight into the catalytic mechanism of non-ribosomal amino acid transferases, peptidyl-RNA conjugates were synthesized for co-crystallization with FemXWv of Weissella viridescens, which transfers L-Ala from Ala-tRNAAla to the peptidoglycan precursor UDP-MurNAc-pentapeptide. The structure of the resulting complex and mutational studies revealed the mechanism by which FemXWv binds its substrates for substrate-assisted catalysis and stabilization of the tetrahedral intermediate.
KW - reaction mechanisms
KW - nucleoprotein complexes
KW - peptidoglycan
KW - peptidyl-RNA conjugates
KW - transferase
UR - http://www.scopus.com/inward/record.url?scp=84880170595&partnerID=8YFLogxK
U2 - 10.1002/anie.201301411
DO - 10.1002/anie.201301411
M3 - Article
C2 - 23744707
AN - SCOPUS:84880170595
SN - 1433-7851
VL - 52
SP - 7278
EP - 7281
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
IS - 28
ER -