The structure of FemXWv in complex with a peptidyl-RNA conjugate: mechanism of aminoacyl transfer from Ala-tRNAAla to peptidoglycan precursors

Matthieu Fonvielle, Inés Li de La Sierra-Gallay, Afaf H. El-Sagheer, Maxime Lecerf, Delphine Patin, Dénia Mellal, Claudine Mayer, Didier Blanot, Nittaya Gale, Tom Brown, Herman van Tilbeurgh, Mélanie Ethève-Quelquejeu, Michel Arthur

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

To gain insight into the catalytic mechanism of non-ribosomal amino acid transferases, peptidyl-RNA conjugates were synthesized for co-crystallization with FemXWv of Weissella viridescens, which transfers L-Ala from Ala-tRNAAla to the peptidoglycan precursor UDP-MurNAc-pentapeptide. The structure of the resulting complex and mutational studies revealed the mechanism by which FemXWv binds its substrates for substrate-assisted catalysis and stabilization of the tetrahedral intermediate.

Original languageEnglish
Pages (from-to)7278-7281
Number of pages4
JournalAngewandte Chemie - International Edition
Volume52
Issue number28
DOIs
Publication statusPublished - 8 Jul 2013
Externally publishedYes

Keywords

  • reaction mechanisms
  • nucleoprotein complexes
  • peptidoglycan
  • peptidyl-RNA conjugates
  • transferase

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