The structure of FemXWv in complex with a peptidyl-RNA conjugate: mechanism of aminoacyl transfer from Ala-tRNAAla to peptidoglycan precursors

Matthieu Fonvielle; Inés Li de La Sierra-Gallay; Afaf H. El-Sagheer; Maxime Lecerf; Delphine Patin; Dénia Mellal; Claudine Mayer; Didier Blanot; Nittaya Gale; Tom Brown; Herman van Tilbeurgh; Mélanie Ethève-Quelquejeu; Michel Arthur

doi:10.1002/anie.201301411

The structure of FemX_Wv in complex with a peptidyl-RNA conjugate: mechanism of aminoacyl transfer from Ala-tRNA^Ala to peptidoglycan precursors

Matthieu Fonvielle, Inés Li de La Sierra-Gallay, Afaf H. El-Sagheer, Maxime Lecerf, Delphine Patin, Dénia Mellal, Claudine Mayer, Didier Blanot, Nittaya Gale, Tom Brown, Herman van Tilbeurgh, Mélanie Ethève-Quelquejeu, Michel Arthur

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

To gain insight into the catalytic mechanism of non-ribosomal amino acid transferases, peptidyl-RNA conjugates were synthesized for co-crystallization with FemX_Wv of Weissella viridescens, which transfers L-Ala from Ala-tRNA^Ala to the peptidoglycan precursor UDP-MurNAc-pentapeptide. The structure of the resulting complex and mutational studies revealed the mechanism by which FemX_Wv binds its substrates for substrate-assisted catalysis and stabilization of the tetrahedral intermediate.

Original language	English
Pages (from-to)	7278-7281
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	52
Issue number	28
DOIs	https://doi.org/10.1002/anie.201301411
Publication status	Published - 8 Jul 2013
Externally published	Yes

Keywords

reaction mechanisms
nucleoprotein complexes
peptidoglycan
peptidyl-RNA conjugates
transferase

Access to Document

10.1002/anie.201301411

Cite this

Fonvielle, M., Li de La Sierra-Gallay, I., El-Sagheer, A. H., Lecerf, M., Patin, D., Mellal, D., Mayer, C., Blanot, D., Gale, N., Brown, T., van Tilbeurgh, H., Ethève-Quelquejeu, M., & Arthur, M. (2013). The structure of FemX_Wv in complex with a peptidyl-RNA conjugate: mechanism of aminoacyl transfer from Ala-tRNA^Ala to peptidoglycan precursors. Angewandte Chemie - International Edition, 52(28), 7278-7281. https://doi.org/10.1002/anie.201301411

@article{99d761c05637409c917d088b919f0bdb,

title = "The structure of FemXWv in complex with a peptidyl-RNA conjugate: mechanism of aminoacyl transfer from Ala-tRNAAla to peptidoglycan precursors",

abstract = "To gain insight into the catalytic mechanism of non-ribosomal amino acid transferases, peptidyl-RNA conjugates were synthesized for co-crystallization with FemXWv of Weissella viridescens, which transfers L-Ala from Ala-tRNAAla to the peptidoglycan precursor UDP-MurNAc-pentapeptide. The structure of the resulting complex and mutational studies revealed the mechanism by which FemXWv binds its substrates for substrate-assisted catalysis and stabilization of the tetrahedral intermediate.",

keywords = "reaction mechanisms, nucleoprotein complexes, peptidoglycan, peptidyl-RNA conjugates, transferase",

author = "Matthieu Fonvielle and \{Li de La Sierra-Gallay\}, In{\'e}s and El-Sagheer, \{Afaf H.\} and Maxime Lecerf and Delphine Patin and D{\'e}nia Mellal and Claudine Mayer and Didier Blanot and Nittaya Gale and Tom Brown and \{van Tilbeurgh\}, Herman and M{\'e}lanie Eth{\`e}ve-Quelquejeu and Michel Arthur",

year = "2013",

month = jul,

day = "8",

doi = "10.1002/anie.201301411",

language = "English",

volume = "52",

pages = "7278--7281",

journal = "Angewandte Chemie - International Edition",

issn = "1433-7851",

publisher = "John Wiley \& Sons",

number = "28",

}

Fonvielle, M, Li de La Sierra-Gallay, I, El-Sagheer, AH, Lecerf, M, Patin, D, Mellal, D, Mayer, C, Blanot, D, Gale, N, Brown, T, van Tilbeurgh, H, Ethève-Quelquejeu, M & Arthur, M 2013, 'The structure of FemX_Wv in complex with a peptidyl-RNA conjugate: mechanism of aminoacyl transfer from Ala-tRNA^Ala to peptidoglycan precursors', Angewandte Chemie - International Edition, vol. 52, no. 28, pp. 7278-7281. https://doi.org/10.1002/anie.201301411

The structure of FemX_Wv in complex with a peptidyl-RNA conjugate: mechanism of aminoacyl transfer from Ala-tRNA^Ala to peptidoglycan precursors. / Fonvielle, Matthieu; Li de La Sierra-Gallay, Inés; El-Sagheer, Afaf H. et al.
In: Angewandte Chemie - International Edition, Vol. 52, No. 28, 08.07.2013, p. 7278-7281.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - The structure of FemXWv in complex with a peptidyl-RNA conjugate

T2 - mechanism of aminoacyl transfer from Ala-tRNAAla to peptidoglycan precursors

AU - Fonvielle, Matthieu

AU - Li de La Sierra-Gallay, Inés

AU - El-Sagheer, Afaf H.

AU - Lecerf, Maxime

AU - Patin, Delphine

AU - Mellal, Dénia

AU - Mayer, Claudine

AU - Blanot, Didier

AU - Gale, Nittaya

AU - Brown, Tom

AU - van Tilbeurgh, Herman

AU - Ethève-Quelquejeu, Mélanie

AU - Arthur, Michel

PY - 2013/7/8

Y1 - 2013/7/8

N2 - To gain insight into the catalytic mechanism of non-ribosomal amino acid transferases, peptidyl-RNA conjugates were synthesized for co-crystallization with FemXWv of Weissella viridescens, which transfers L-Ala from Ala-tRNAAla to the peptidoglycan precursor UDP-MurNAc-pentapeptide. The structure of the resulting complex and mutational studies revealed the mechanism by which FemXWv binds its substrates for substrate-assisted catalysis and stabilization of the tetrahedral intermediate.

AB - To gain insight into the catalytic mechanism of non-ribosomal amino acid transferases, peptidyl-RNA conjugates were synthesized for co-crystallization with FemXWv of Weissella viridescens, which transfers L-Ala from Ala-tRNAAla to the peptidoglycan precursor UDP-MurNAc-pentapeptide. The structure of the resulting complex and mutational studies revealed the mechanism by which FemXWv binds its substrates for substrate-assisted catalysis and stabilization of the tetrahedral intermediate.

KW - reaction mechanisms

KW - nucleoprotein complexes

KW - peptidoglycan

KW - peptidyl-RNA conjugates

KW - transferase

UR - https://www.scopus.com/pages/publications/84880170595

U2 - 10.1002/anie.201301411

DO - 10.1002/anie.201301411

M3 - Article

C2 - 23744707

AN - SCOPUS:84880170595

SN - 1433-7851

VL - 52

SP - 7278

EP - 7281

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 28

ER -

The structure of FemX_Wv in complex with a peptidyl-RNA conjugate: mechanism of aminoacyl transfer from Ala-tRNA^Ala to peptidoglycan precursors

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this