The synthesis and characterisation of a novel dendritic system for gene delivery

Daniel J. Coles; Shu Yang; Anna Esposito; Deanne Mitchell; Rodney F. Minchin; Istvan Toth

doi:10.1016/j.tet.2007.09.048

The synthesis and characterisation of a novel dendritic system for gene delivery

Daniel J. Coles, Shu Yang, Anna Esposito, Deanne Mitchell, Rodney F. Minchin, Istvan Toth^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

There has been increasing interest in recent years in gene delivery. We report the synthesis of non-viral delivery systems composed of variations of the cell penetrating peptide TAT, a nuclear localisation signal peptide and dendritic polylysine. The delivery systems were tested for their ability to form complexes with plasmid DNA by utilising gel shift analysis, isothermal titration calorimetry, particle size analysis, zeta potential and transmission electron microscopy. These techniques indicated the successful formation of complexes between the peptide dendrimer and DNA.

Original language	English
Pages (from-to)	12207-12214
Number of pages	8
Journal	Tetrahedron
Volume	63
Issue number	49
DOIs	https://doi.org/10.1016/j.tet.2007.09.048
Publication status	Published - 3 Dec 2007
Externally published	Yes

Keywords

dendrimer
gene delivery
NLS
TAT

Access to Document

10.1016/j.tet.2007.09.048

Cite this

@article{56b2676b770947b7a471900c45b1ea36,

title = "The synthesis and characterisation of a novel dendritic system for gene delivery",

abstract = "There has been increasing interest in recent years in gene delivery. We report the synthesis of non-viral delivery systems composed of variations of the cell penetrating peptide TAT, a nuclear localisation signal peptide and dendritic polylysine. The delivery systems were tested for their ability to form complexes with plasmid DNA by utilising gel shift analysis, isothermal titration calorimetry, particle size analysis, zeta potential and transmission electron microscopy. These techniques indicated the successful formation of complexes between the peptide dendrimer and DNA.",

keywords = "dendrimer, gene delivery, NLS, TAT",

author = "Coles, {Daniel J.} and Shu Yang and Anna Esposito and Deanne Mitchell and Minchin, {Rodney F.} and Istvan Toth",

year = "2007",

month = dec,

day = "3",

doi = "10.1016/j.tet.2007.09.048",

language = "English",

volume = "63",

pages = "12207--12214",

journal = "Tetrahedron",

issn = "0040-4020",

publisher = "Elsevier",

number = "49",

}

TY - JOUR

T1 - The synthesis and characterisation of a novel dendritic system for gene delivery

AU - Coles, Daniel J.

AU - Yang, Shu

AU - Esposito, Anna

AU - Mitchell, Deanne

AU - Minchin, Rodney F.

AU - Toth, Istvan

PY - 2007/12/3

Y1 - 2007/12/3

N2 - There has been increasing interest in recent years in gene delivery. We report the synthesis of non-viral delivery systems composed of variations of the cell penetrating peptide TAT, a nuclear localisation signal peptide and dendritic polylysine. The delivery systems were tested for their ability to form complexes with plasmid DNA by utilising gel shift analysis, isothermal titration calorimetry, particle size analysis, zeta potential and transmission electron microscopy. These techniques indicated the successful formation of complexes between the peptide dendrimer and DNA.

AB - There has been increasing interest in recent years in gene delivery. We report the synthesis of non-viral delivery systems composed of variations of the cell penetrating peptide TAT, a nuclear localisation signal peptide and dendritic polylysine. The delivery systems were tested for their ability to form complexes with plasmid DNA by utilising gel shift analysis, isothermal titration calorimetry, particle size analysis, zeta potential and transmission electron microscopy. These techniques indicated the successful formation of complexes between the peptide dendrimer and DNA.

KW - dendrimer

KW - gene delivery

KW - NLS

KW - TAT

UR - http://www.scopus.com/inward/record.url?scp=35348970844&partnerID=8YFLogxK

U2 - 10.1016/j.tet.2007.09.048

DO - 10.1016/j.tet.2007.09.048

M3 - Article

AN - SCOPUS:35348970844

SN - 0040-4020

VL - 63

SP - 12207

EP - 12214

JO - Tetrahedron

JF - Tetrahedron

IS - 49

ER -

The synthesis and characterisation of a novel dendritic system for gene delivery

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this