The Therapeutic implications of FeAu nanoparticles in head and neck cancer chemotherapy and the molecular mechanism of selective anti-neoplastic efficacy

D.-B. Shieh, Y.-N. Wu, D.-H. Chen, C.-S. Yeh, K. Ratinac, F. Braet

Research output: Contribution to journalMeeting abstract

Abstract

Gold and iron were processed to form core shell (gold coated on iron, Fe@Au) or alloy nanoparticles and transferred to aqueous phase by ethanol rinse and magnetic separation. Both types of nanoparticles presented selective and significant (P <0.0001) inhibition of head and neck cancer cell proliferation in vitro at doses as low as 5 μg/mL, while having little adverse effect on normal healthy control cells. The Fe@Au particle treatment induced delay in cell-cycle progression, especially in the S-phase. There was no significant difference in the nanoparticle uptake between cancer and control cells. The cytotoxicity resulted primarily from the iron core, before oxidation, rather than the Fe ions released from the core. The nanoparticles triggered lost of mitochondria membrane potential in cancerous cells but not in normal cells. The nanoparticles do not induce significant apoptotic response but autophagy was detected from both ultrastructural observation and immunoblot of markers. The nanoparticles presented therapeutic efficacy in cancer bearing animal and a significant synergistic effect was found when co-treat with anti-cancer drug methotrexate. The Fe@Au nanoparticle showed better anti-cancer effect than the FeAu alloy nanoparticle in the same dosage. In contrast to most magnetic nanoparticles for medical application that usually serve as drug carriers or diagnostic probes or hyperthermia energy transducer, the FeAu nanoparticles selectively suppressed cancer cell growth and left healthy control cells unaffected in vitro and in vivo. This novel nanomaterial holds great promise as a therapeutic tool in nanomedicine.
Original languageEnglish
Pages (from-to)S74-S74
Number of pages1
JournalOral oncology, volume 47 supplement 1 : third world congress of the International Academy of Oral Oncology 2011
Volume47
DOIs
Publication statusPublished - 2011
Externally publishedYes
EventWorld congress of the International Academy of Oral Oncology (3rd : 2011) - Singapore
Duration: 14 Jul 201117 Jul 2011

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