The thermostabilizing domain, XynA, of Caldibacillus cellulovorans xylanase is a xylan binding domain

Anwar Sunna; Moreland D. Gibbs; Peter L. Bergquist

doi:10.1042/0264-6021:3460583

The thermostabilizing domain, XynA, of Caldibacillus cellulovorans xylanase is a xylan binding domain

Anwar Sunna, Moreland D. Gibbs, Peter L. Bergquist^*

^*Corresponding author for this work

Macquarie University Administration Unit

Research output: Contribution to journal › Article › peer-review

42 Citations (Scopus)

Abstract

We show that the N-terminal 'thermostabilizing domain' (TSD) of the xylanase, XynA, from the thermophilic bacterium Caldibacillus cellulovorans also acts as a xylan binding domain. Affinity electrophoresis experiments show that this TSD selectively binds soluble xylan and binds weakly to hydroxyethylcellulose. Based on this, and previously reported evidence, we propose that xylanase-associated TSDs are xylan binding domains.

Original language	English
Pages (from-to)	583-586
Number of pages	4
Journal	Biochemical Journal
Volume	346
Issue number	3
DOIs	https://doi.org/10.1042/0264-6021:3460583
Publication status	Published - 15 Mar 2000

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abstract = "We show that the N-terminal 'thermostabilizing domain' (TSD) of the xylanase, XynA, from the thermophilic bacterium Caldibacillus cellulovorans also acts as a xylan binding domain. Affinity electrophoresis experiments show that this TSD selectively binds soluble xylan and binds weakly to hydroxyethylcellulose. Based on this, and previously reported evidence, we propose that xylanase-associated TSDs are xylan binding domains.",

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AB - We show that the N-terminal 'thermostabilizing domain' (TSD) of the xylanase, XynA, from the thermophilic bacterium Caldibacillus cellulovorans also acts as a xylan binding domain. Affinity electrophoresis experiments show that this TSD selectively binds soluble xylan and binds weakly to hydroxyethylcellulose. Based on this, and previously reported evidence, we propose that xylanase-associated TSDs are xylan binding domains.

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The thermostabilizing domain, XynA, of Caldibacillus cellulovorans xylanase is a xylan binding domain

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