The thermostabilizing domain, XynA, of Caldibacillus cellulovorans xylanase is a xylan binding domain

Anwar Sunna, Moreland D. Gibbs, Peter L. Bergquist

Research output: Contribution to journalArticleResearchpeer-review

Abstract

We show that the N-terminal 'thermostabilizing domain' (TSD) of the xylanase, XynA, from the thermophilic bacterium Caldibacillus cellulovorans also acts as a xylan binding domain. Affinity electrophoresis experiments show that this TSD selectively binds soluble xylan and binds weakly to hydroxyethylcellulose. Based on this, and previously reported evidence, we propose that xylanase-associated TSDs are xylan binding domains.

LanguageEnglish
Pages583-586
Number of pages4
JournalBiochemical Journal
Volume346
Issue number3
DOIs
Publication statusPublished - 15 Mar 2000

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Xylans
Electrophoresis
Bacteria
Experiments

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Sunna, Anwar ; Gibbs, Moreland D. ; Bergquist, Peter L. / The thermostabilizing domain, XynA, of Caldibacillus cellulovorans xylanase is a xylan binding domain. In: Biochemical Journal. 2000 ; Vol. 346, No. 3. pp. 583-586.
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The thermostabilizing domain, XynA, of Caldibacillus cellulovorans xylanase is a xylan binding domain. / Sunna, Anwar; Gibbs, Moreland D.; Bergquist, Peter L.

In: Biochemical Journal, Vol. 346, No. 3, 15.03.2000, p. 583-586.

Research output: Contribution to journalArticleResearchpeer-review

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