The thermostabilizing domain, XynA, of Caldibacillus cellulovorans xylanase is a xylan binding domain

Anwar Sunna, Moreland D. Gibbs, Peter L. Bergquist*

*Corresponding author for this work

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

We show that the N-terminal 'thermostabilizing domain' (TSD) of the xylanase, XynA, from the thermophilic bacterium Caldibacillus cellulovorans also acts as a xylan binding domain. Affinity electrophoresis experiments show that this TSD selectively binds soluble xylan and binds weakly to hydroxyethylcellulose. Based on this, and previously reported evidence, we propose that xylanase-associated TSDs are xylan binding domains.

Original languageEnglish
Pages (from-to)583-586
Number of pages4
JournalBiochemical Journal
Volume346
Issue number3
DOIs
Publication statusPublished - 15 Mar 2000

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