The transcriptome analysis of strongyloides stercoralis L3i larvae reveals targets for intervention in a neglected disease

Antonio Marcilla*, Gagan Garg, Dolores Bernal, Shoba Ranganathan, Javier Forment, Javier Ortiz, Carla Muñoz-Antolí, M. Victoria Dominguez, Laia Pedrola, Juan Martinez-Blanch, Javier Sotillo, Maria Trelis, Rafael Toledo, J. Guillermo Esteban

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)
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Background: Strongyloidiasis is one of the most neglected diseases distributed worldwide with endemic areas in developed countries, where chronic infections are life threatening. Despite its impact, very little is known about the molecular biology of the parasite involved and its interplay with its hosts. Next generation sequencing technologies now provide unique opportunities to rapidly address these questions. Principal Findings: Here we present the first transcriptome of the third larval stage of S. stercoralis using 454 sequencing coupled with semi-automated bioinformatic analyses. 253,266 raw sequence reads were assembled into 11,250 contiguous sequences, most of which were novel. 8037 putative proteins were characterized based on homology, gene ontology and/or biochemical pathways. Comparison of the transcriptome of S. strongyloides with those of other nematodes, including S. ratti, revealed similarities in transcription of molecules inferred to have key roles in parasite-host interactions. Enzymatic proteins, like kinases and proteases, were abundant. 1213 putative excretory/secretory proteins were compiled using a new pipeline which included non-classical secretory proteins. Potential drug targets were also identified. Conclusions: Overall, the present dataset should provide a solid foundation for future fundamental genomic, proteomic and metabolomic explorations of S. stercoralis, as well as a basis for applied outcomes, such as the development of novel methods of intervention against this neglected parasite.

Original languageEnglish
Article numbere1513
Pages (from-to)1-9
Number of pages9
JournalPLoS Neglected Tropical Diseases
Issue number2
Publication statusPublished - Feb 2012

Bibliographical note

Copyright the Author(s) 2012. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.


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