TY - JOUR
T1 - The trypsin inhibitor pro-peptide induces toxic effects in Indianmeal moth, Plodia interpunctella
AU - Hemmati, Seyed Ali
AU - Takalloo, Zeinab
AU - Taghdir, Majid
AU - Mehrabadi, Mohammad
AU - Balalaei, Saeed
AU - Moharramipour, Saeid
AU - Sajedi, Reza H.
PY - 2021/1
Y1 - 2021/1
N2 - The inhibitory potential of an inhibitor peptide based on the pro-region of trypsin zymogen was investigated in Indianmeal moth, P. interpunctella, which is a world-wide insect pest of stored food. Five peptides were designed based on molecular docking simulations. The designed peptide with the best score was selected and synthesized for further screening in vitro and in vivo. The peptide was characterized and its inhibitory effects towards the insect trypsin were evaluated and the kinetic analysis revealed a competitive type of inhibition against the target enzyme. The results showed that the peptide could successfully suppress the pest midgut trypsin, and more interestingly, it did not show considerable inhibitory effects on a mammalian trypsin. We also aimed to assess the effect of dietary insect meal treated with different concentrations of the peptide and observed a significant growth and development retardation in pupa and adult insects fed with the inhibitor peptide. The outcomes of the present study suggest an efficient inhibitor peptide that could specifically bind the P. interpunctella trypsin and inhibit its activity, which would be safe against human being health and environment. Notably, this is the first report on in vivo assessment of the direct effect of a pro-region as the specific inhibitor in development as well as survival of the pest insect. Furthermore, our findings could be a promising for future designed pesticides used in pest management.
AB - The inhibitory potential of an inhibitor peptide based on the pro-region of trypsin zymogen was investigated in Indianmeal moth, P. interpunctella, which is a world-wide insect pest of stored food. Five peptides were designed based on molecular docking simulations. The designed peptide with the best score was selected and synthesized for further screening in vitro and in vivo. The peptide was characterized and its inhibitory effects towards the insect trypsin were evaluated and the kinetic analysis revealed a competitive type of inhibition against the target enzyme. The results showed that the peptide could successfully suppress the pest midgut trypsin, and more interestingly, it did not show considerable inhibitory effects on a mammalian trypsin. We also aimed to assess the effect of dietary insect meal treated with different concentrations of the peptide and observed a significant growth and development retardation in pupa and adult insects fed with the inhibitor peptide. The outcomes of the present study suggest an efficient inhibitor peptide that could specifically bind the P. interpunctella trypsin and inhibit its activity, which would be safe against human being health and environment. Notably, this is the first report on in vivo assessment of the direct effect of a pro-region as the specific inhibitor in development as well as survival of the pest insect. Furthermore, our findings could be a promising for future designed pesticides used in pest management.
KW - Inhibitor peptide
KW - Midgut trypsin
KW - Pest management
KW - Plodia interpunctella
UR - http://www.scopus.com/inward/record.url?scp=85094563004&partnerID=8YFLogxK
U2 - 10.1016/j.pestbp.2020.104730
DO - 10.1016/j.pestbp.2020.104730
M3 - Article
C2 - 33357552
SN - 0048-3575
VL - 171
SP - 1
EP - 11
JO - Pesticide Biochemistry and Physiology
JF - Pesticide Biochemistry and Physiology
M1 - 104730
ER -