The unique 16S rRNA genes of piezophiles reflect both phylogeny and adaptation

Federico M. Lauro, Roger A. Chastain, Lesley E. Blankenship, A. Aristides Yayanos, Douglas H. Bartlett*

*Corresponding author for this work

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

In the ocean's most extreme depths, pressures of 70 to 110 megapascals prevent the growth of all but the most liyperpiezophilic (pressure-loving) organisms. The physiological adaptations required for growth under these conditions are considered to be substantial. Efforts to determine specific adaptations permitting growth at extreme pressures have thus far focused on relatively few γ-proteobacteria, in part due to the technical difficulties of obtaining piezophilic bacteria in pure culture. Here, we present the molecular phylogenies of several new piezophiles of widely differing geographic origins. Included are results from an analysis of the first deep-trench bacterial isolates recovered from the southern hemisphere (9.9-km depth) and of the first gram-positive piezophilic strains. These new data allowed both phylogenetic and structural 16S rRNA comparisons among deep-ocean trench piezophiles and closely related strains not adapted to high pressure. Our results suggest that (i) the Circumpolar Deep Water acts as repository for liyperpiezophiles and drives their dissemination to deep trenches in the Pacific Ocean and (ii) the occurrence of elongated helices in the 16S rRNA genes increases with the extent of adaptation to growth at elevated pressure. These helix changes are believed to improve ribosome function under deep-sea conditions.

Original languageEnglish
Pages (from-to)838-845
Number of pages8
JournalApplied and Environmental Microbiology
Volume73
Issue number3
DOIs
Publication statusPublished - Feb 2007
Externally publishedYes

Fingerprint Dive into the research topics of 'The unique 16S rRNA genes of piezophiles reflect both phylogeny and adaptation'. Together they form a unique fingerprint.

  • Cite this

    Lauro, F. M., Chastain, R. A., Blankenship, L. E., Yayanos, A. A., & Bartlett, D. H. (2007). The unique 16S rRNA genes of piezophiles reflect both phylogeny and adaptation. Applied and Environmental Microbiology, 73(3), 838-845. https://doi.org/10.1128/AEM.01726-06