The use of microfluidic neuronal devices to study the anterograde axonal transport of herpes simplex virus-1

Kevin Danastas, Anthony L. Cunningham, Monica Miranda-Saksena

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

3 Citations (Scopus)

Abstract

Understanding how herpes simplex virus-1 (HSV-1) interacts with different parts of the neuron is fundamental in understanding the mechanisms behind HSV-1 transport during primary and recurrent infections. In this chapter, we describe a unique neuronal culture system that is capable of compartmentalizing neuronal cell bodies from their axons to study the transport of HSV-1 along axons. The ability to separate neuronal cell bodies and axons provides a unique model to investigate the mechanisms used by HSV-1 for viral transport, assembly, and exit from different parts of the neuron.

Original languageEnglish
Title of host publicationHerpes Simplex Virus
Subtitle of host publicationMethods and Protocols
EditorsRussell J. Diefenbach, Cornel Fraefel
Place of PublicationNew York, NY
PublisherSpringer, Springer Nature
Chapter25
Pages409-418
Number of pages10
ISBN (Electronic)9781493998142
ISBN (Print)9781493998135
DOIs
Publication statusPublished - 2020
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume2060
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Axons
  • Herpes simplex virus
  • Microfluidics
  • Neurons
  • Transport

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