The use of microinjected colloidal gold and immunocytochemistry to localise pressor sites in the rostral medulla oblongata of the rat

Anthony Hodgson*, Jane Minson, John Chalmers, Paul Pilowsky

*Corresponding author for this work

    Research output: Contribution to journalArticle

    17 Citations (Scopus)

    Abstract

    A new technique for localising the centre of sites of microinjection of drugs in the brain using the silver intensification of colloidal gold is described. The technique is compatible with immunocytochemistry and in this study was used in Wistar Kyoto rats to investigate the relationship between the location of phenylethanolamine-N- methyltransferase-like immunoreactive (PNMT-LI) cell bodies in the medulla and the precise sites where microinjection of l-glutamate elicited pressor responses. Colloidal gold provided a simple technique for localising the centre of sites of microinjection with a high degree of spatial resolution and allowed pressor sites to be precisely localised in the region of ventrolateral medulla with the highest density of PNMT-LI cells.

    Original languageEnglish
    Pages (from-to)125-130
    Number of pages6
    JournalNeuroscience Letters
    Volume77
    Issue number2
    DOIs
    Publication statusPublished - 15 Jun 1987

    Keywords

    • Blood pressure
    • Colloidal gold
    • Glutamate
    • Medulla oblongata
    • Microinjection of drugs
    • Phenylethanolamine-N-methyltransferase
    • Rat

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