The use of polymorphic DNA and protein markers for the third complement component for determining linkage of familial hypercholesterolaemia

S. E. Humphries*, J. A. Donald, J. J P McFadden, S. Shull, R. Williamson, N. I. Jowett, D. J. Galton, J. O. Julsrud, K. Berg, A. Heiberg, S. Ball, G. Fey, M. Seed, V. Wynn

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

We have used DNA and protein polymorphisms for the third complement component (C3) to assess the potential of DNA markers in the diagnosis and study of familial hypercholesterolaemia (FH), and to confirm the reported linkage between FH and C3. The inheritance of FH and the C3 gene has been studied in 10 families by combining information from both the protein and DNA polymorphisms. Our results confirm that the C3 gene is loosely linked to the gene causing FH (lod score maximum of 2.0) at a recombination distance of 0.15. When these results are combined with previously published data the overall lod score maximum is 4.75 at a recombination distance of 0.2, meaning that the two genes will be inherited together in only about 80% of children. These results confirm that the gene that causes familial hypercholesterolaemia is linked to C3 and is therefore on chromosome 19, but C3 is not close enough to be used as a diagnostic marker.

Original languageEnglish
Pages (from-to)267-278
Number of pages12
JournalAtherosclerosis
Volume52
Issue number3
DOIs
Publication statusPublished - 1984
Externally publishedYes

Keywords

  • C3
  • DNA polymorphic marker
  • Familial hypercholesterolaemia
  • Linkage
  • Southern blots

Fingerprint

Dive into the research topics of 'The use of polymorphic DNA and protein markers for the third complement component for determining linkage of familial hypercholesterolaemia'. Together they form a unique fingerprint.

Cite this