The use of solid-phase extraction with graphitised carbon for the fractionation and purification of sugars

John W. Redmond, Nicolle H. Packer*

*Corresponding author for this work

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The unique selectivity, high preparative capacity and chemical inertness of graphitised carbon make it an ideal medium for the solid-phase extraction (SPE) of sugars from dilute solution, and for their analytical and preparative separation from salt, alkali or mineral acid. Graded elution with water containing an organic modifier (such as an alcohol or acetonitrile) permits the separation of groups of oligosaccharides. Acidic sugars are retained by graphitised carbon, while comparable neutral and amino sugars are eluted by water containing an organic modifier; the acidic components are then eluted by the same eluant containing trifluoroacetic acid. As such, graphitised carbon presents a much-needed solid-phase packing for the general clean-up and separation of sugars. Copyright (C) 1999 Elsevier Science Ltd.

Original languageEnglish
Pages (from-to)74-79
Number of pages6
JournalCarbohydrate Research
Volume319
Issue number1-4
DOIs
Publication statusPublished - 30 Jun 1999

Keywords

  • Chromatography
  • Graphitised carbon
  • Solid-phase extraction

Fingerprint Dive into the research topics of 'The use of solid-phase extraction with graphitised carbon for the fractionation and purification of sugars'. Together they form a unique fingerprint.

  • Cite this