The use of starch and skim milk in the regeneration of Bacillus amylotiquefaciens and Bacillus subtilis protoplasts

Y. B. BHATT*, V. R. SUBRAMANYAM, F. F. DIAS

*Corresponding author for this work

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The addition of starch or skim milk to bovine serum albumin‐containing regeneration medium enhances the regeneration of both Bacillus amyloliquefaciens and B. subtilis protoplasts. With B. amyloliquefaciens starch could replace bovine serum albumin; with B. subtilis either starch or skim milk could be used instead of bovine serum albumin. The maximum regeneration frequency achieved was 10% with B. amyloliquefaciens and 290% with B. subtilis.

Original languageEnglish
Pages (from-to)77-79
Number of pages3
JournalLetters in Applied Microbiology
Volume4
Issue number4
DOIs
Publication statusPublished - 1987
Externally publishedYes

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