The use of starch and skim milk in the regeneration of Bacillus amylotiquefaciens and Bacillus subtilis protoplasts

Y. B. BHATT; V. R. SUBRAMANYAM; F. F. DIAS

doi:10.1111/j.1472-765X.1987.tb01587.x

The use of starch and skim milk in the regeneration of Bacillus amylotiquefaciens and Bacillus subtilis protoplasts

Y. B. BHATT^*, V. R. SUBRAMANYAM, F. F. DIAS

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

The addition of starch or skim milk to bovine serum albumin‐containing regeneration medium enhances the regeneration of both Bacillus amyloliquefaciens and B. subtilis protoplasts. With B. amyloliquefaciens starch could replace bovine serum albumin; with B. subtilis either starch or skim milk could be used instead of bovine serum albumin. The maximum regeneration frequency achieved was 10% with B. amyloliquefaciens and 290% with B. subtilis.

Original language	English
Pages (from-to)	77-79
Number of pages	3
Journal	Letters in Applied Microbiology
Volume	4
Issue number	4
DOIs	https://doi.org/10.1111/j.1472-765X.1987.tb01587.x
Publication status	Published - 1987
Externally published	Yes

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title = "The use of starch and skim milk in the regeneration of Bacillus amylotiquefaciens and Bacillus subtilis protoplasts",

abstract = "The addition of starch or skim milk to bovine serum albumin‐containing regeneration medium enhances the regeneration of both Bacillus amyloliquefaciens and B. subtilis protoplasts. With B. amyloliquefaciens starch could replace bovine serum albumin; with B. subtilis either starch or skim milk could be used instead of bovine serum albumin. The maximum regeneration frequency achieved was 10% with B. amyloliquefaciens and 290% with B. subtilis.",

author = "BHATT, {Y. B.} and SUBRAMANYAM, {V. R.} and DIAS, {F. F.}",

year = "1987",

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AU - SUBRAMANYAM, V. R.

AU - DIAS, F. F.

PY - 1987

Y1 - 1987

N2 - The addition of starch or skim milk to bovine serum albumin‐containing regeneration medium enhances the regeneration of both Bacillus amyloliquefaciens and B. subtilis protoplasts. With B. amyloliquefaciens starch could replace bovine serum albumin; with B. subtilis either starch or skim milk could be used instead of bovine serum albumin. The maximum regeneration frequency achieved was 10% with B. amyloliquefaciens and 290% with B. subtilis.

AB - The addition of starch or skim milk to bovine serum albumin‐containing regeneration medium enhances the regeneration of both Bacillus amyloliquefaciens and B. subtilis protoplasts. With B. amyloliquefaciens starch could replace bovine serum albumin; with B. subtilis either starch or skim milk could be used instead of bovine serum albumin. The maximum regeneration frequency achieved was 10% with B. amyloliquefaciens and 290% with B. subtilis.

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U2 - 10.1111/j.1472-765X.1987.tb01587.x

DO - 10.1111/j.1472-765X.1987.tb01587.x

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JO - Letters in Applied Microbiology

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IS - 4

ER -

The use of starch and skim milk in the regeneration of Bacillus amylotiquefaciens and Bacillus subtilis protoplasts

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