Thermo-responsive polymeric nanoparticles for enhancing neuronal differentiation of human induced pluripotent stem cells

Hye In Seo, Ann-Na Cho, Jiho Jang, Dong-Wook Kim, Seung-Woo Cho, Bong Geun Chung

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

We report thermo-responsive retinoic acid (RA)-loaded poly(N-isopropylacrylamide)-co-acrylamide (PNIPAM-co-Am) nanoparticles for directing human induced pluripotent stem cell (hiPSC) fate. Fourier transform infrared spectroscopy and 1H nuclear magnetic resonance analysis confirmed that RA was efficiently incorporated into PNIAPM-co-Am nanoparticles (PCANs). The size of PCANs dropped with increasing temperatures (300-400 nm at room temperature, 80-90 nm at 37 °C) due to its phase transition from hydrophilic to hydrophobic. Due to particle shrinkage caused by this thermo-responsive property of PCANs, RA could be released from nanoparticles in the cells upon cellular uptake. Immunocytochemistry and quantitative real-time polymerase chain reaction analysis demonstrated that neuronal differentiation of hiPSC-derived neuronal precursors was enhanced after treatment with 1-2 μg/ml RA-loaded PCANs. Therefore, we propose that this PCAN could be a potentially powerful carrier for effective RA delivery to direct hiPSC fate to neuronal lineage.
Original languageEnglish
Pages (from-to)1861-1869
Number of pages9
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume11
Issue number7
DOIs
Publication statusPublished - Oct 2015
Externally publishedYes

Keywords

  • Thermo-responsive nanoparticle
  • Poly(N-isopropylacrylamide)-co-acrylamide
  • Retinoic acid
  • Human induced pluripotent stem cells
  • Neuronal differentiation

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