Thermo-responsive polymeric nanoparticles for enhancing neuronal differentiation of human induced pluripotent stem cells

Hye In Seo; Ann-Na Cho; Jiho Jang; Dong-Wook Kim; Seung-Woo Cho; Bong Geun Chung

doi:10.1016/j.nano.2015.05.008

Thermo-responsive polymeric nanoparticles for enhancing neuronal differentiation of human induced pluripotent stem cells

Hye In Seo, Ann-Na Cho, Jiho Jang, Dong-Wook Kim, Seung-Woo Cho, Bong Geun Chung

Research output: Contribution to journal › Article › peer-review

38 Citations (Scopus)

Abstract

We report thermo-responsive retinoic acid (RA)-loaded poly(N-isopropylacrylamide)-co-acrylamide (PNIPAM-co-Am) nanoparticles for directing human induced pluripotent stem cell (hiPSC) fate. Fourier transform infrared spectroscopy and 1H nuclear magnetic resonance analysis confirmed that RA was efficiently incorporated into PNIAPM-co-Am nanoparticles (PCANs). The size of PCANs dropped with increasing temperatures (300-400 nm at room temperature, 80-90 nm at 37 °C) due to its phase transition from hydrophilic to hydrophobic. Due to particle shrinkage caused by this thermo-responsive property of PCANs, RA could be released from nanoparticles in the cells upon cellular uptake. Immunocytochemistry and quantitative real-time polymerase chain reaction analysis demonstrated that neuronal differentiation of hiPSC-derived neuronal precursors was enhanced after treatment with 1-2 μg/ml RA-loaded PCANs. Therefore, we propose that this PCAN could be a potentially powerful carrier for effective RA delivery to direct hiPSC fate to neuronal lineage.

Original language	English
Pages (from-to)	1861-1869
Number of pages	9
Journal	Nanomedicine: Nanotechnology, Biology, and Medicine
Volume	11
Issue number	7
DOIs	https://doi.org/10.1016/j.nano.2015.05.008
Publication status	Published - Oct 2015
Externally published	Yes

Keywords

Thermo-responsive nanoparticle
Poly(N-isopropylacrylamide)-co-acrylamide
Retinoic acid
Human induced pluripotent stem cells
Neuronal differentiation

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10.1016/j.nano.2015.05.008

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title = "Thermo-responsive polymeric nanoparticles for enhancing neuronal differentiation of human induced pluripotent stem cells",

abstract = "We report thermo-responsive retinoic acid (RA)-loaded poly(N-isopropylacrylamide)-co-acrylamide (PNIPAM-co-Am) nanoparticles for directing human induced pluripotent stem cell (hiPSC) fate. Fourier transform infrared spectroscopy and 1H nuclear magnetic resonance analysis confirmed that RA was efficiently incorporated into PNIAPM-co-Am nanoparticles (PCANs). The size of PCANs dropped with increasing temperatures (300-400 nm at room temperature, 80-90 nm at 37 °C) due to its phase transition from hydrophilic to hydrophobic. Due to particle shrinkage caused by this thermo-responsive property of PCANs, RA could be released from nanoparticles in the cells upon cellular uptake. Immunocytochemistry and quantitative real-time polymerase chain reaction analysis demonstrated that neuronal differentiation of hiPSC-derived neuronal precursors was enhanced after treatment with 1-2 μg/ml RA-loaded PCANs. Therefore, we propose that this PCAN could be a potentially powerful carrier for effective RA delivery to direct hiPSC fate to neuronal lineage.",

keywords = "Thermo-responsive nanoparticle, Poly(N-isopropylacrylamide)-co-acrylamide, Retinoic acid, Human induced pluripotent stem cells, Neuronal differentiation",

author = "Seo, {Hye In} and Ann-Na Cho and Jiho Jang and Dong-Wook Kim and Seung-Woo Cho and Chung, {Bong Geun}",

year = "2015",

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doi = "10.1016/j.nano.2015.05.008",

language = "English",

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pages = "1861--1869",

journal = "Nanomedicine: Nanotechnology, Biology, and Medicine",

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T1 - Thermo-responsive polymeric nanoparticles for enhancing neuronal differentiation of human induced pluripotent stem cells

AU - Seo, Hye In

AU - Cho, Ann-Na

AU - Jang, Jiho

AU - Kim, Dong-Wook

AU - Cho, Seung-Woo

AU - Chung, Bong Geun

PY - 2015/10

Y1 - 2015/10

N2 - We report thermo-responsive retinoic acid (RA)-loaded poly(N-isopropylacrylamide)-co-acrylamide (PNIPAM-co-Am) nanoparticles for directing human induced pluripotent stem cell (hiPSC) fate. Fourier transform infrared spectroscopy and 1H nuclear magnetic resonance analysis confirmed that RA was efficiently incorporated into PNIAPM-co-Am nanoparticles (PCANs). The size of PCANs dropped with increasing temperatures (300-400 nm at room temperature, 80-90 nm at 37 °C) due to its phase transition from hydrophilic to hydrophobic. Due to particle shrinkage caused by this thermo-responsive property of PCANs, RA could be released from nanoparticles in the cells upon cellular uptake. Immunocytochemistry and quantitative real-time polymerase chain reaction analysis demonstrated that neuronal differentiation of hiPSC-derived neuronal precursors was enhanced after treatment with 1-2 μg/ml RA-loaded PCANs. Therefore, we propose that this PCAN could be a potentially powerful carrier for effective RA delivery to direct hiPSC fate to neuronal lineage.

AB - We report thermo-responsive retinoic acid (RA)-loaded poly(N-isopropylacrylamide)-co-acrylamide (PNIPAM-co-Am) nanoparticles for directing human induced pluripotent stem cell (hiPSC) fate. Fourier transform infrared spectroscopy and 1H nuclear magnetic resonance analysis confirmed that RA was efficiently incorporated into PNIAPM-co-Am nanoparticles (PCANs). The size of PCANs dropped with increasing temperatures (300-400 nm at room temperature, 80-90 nm at 37 °C) due to its phase transition from hydrophilic to hydrophobic. Due to particle shrinkage caused by this thermo-responsive property of PCANs, RA could be released from nanoparticles in the cells upon cellular uptake. Immunocytochemistry and quantitative real-time polymerase chain reaction analysis demonstrated that neuronal differentiation of hiPSC-derived neuronal precursors was enhanced after treatment with 1-2 μg/ml RA-loaded PCANs. Therefore, we propose that this PCAN could be a potentially powerful carrier for effective RA delivery to direct hiPSC fate to neuronal lineage.

KW - Thermo-responsive nanoparticle

KW - Poly(N-isopropylacrylamide)-co-acrylamide

KW - Retinoic acid

KW - Human induced pluripotent stem cells

KW - Neuronal differentiation

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DO - 10.1016/j.nano.2015.05.008

M3 - Article

C2 - 26093056

VL - 11

SP - 1861

EP - 1869

JO - Nanomedicine: Nanotechnology, Biology, and Medicine

JF - Nanomedicine: Nanotechnology, Biology, and Medicine

SN - 1549-9634

IS - 7

ER -

Thermo-responsive polymeric nanoparticles for enhancing neuronal differentiation of human induced pluripotent stem cells

Abstract

Keywords

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