Abstract
We report thermo-responsive retinoic acid (RA)-loaded poly(N-isopropylacrylamide)-co-acrylamide (PNIPAM-co-Am) nanoparticles for directing human induced pluripotent stem cell (hiPSC) fate. Fourier transform infrared spectroscopy and 1H nuclear magnetic resonance analysis confirmed that RA was efficiently incorporated into PNIAPM-co-Am nanoparticles (PCANs). The size of PCANs dropped with increasing temperatures (300-400 nm at room temperature, 80-90 nm at 37 °C) due to its phase transition from hydrophilic to hydrophobic. Due to particle shrinkage caused by this thermo-responsive property of PCANs, RA could be released from nanoparticles in the cells upon cellular uptake. Immunocytochemistry and quantitative real-time polymerase chain reaction analysis demonstrated that neuronal differentiation of hiPSC-derived neuronal precursors was enhanced after treatment with 1-2 μg/ml RA-loaded PCANs. Therefore, we propose that this PCAN could be a potentially powerful carrier for effective RA delivery to direct hiPSC fate to neuronal lineage.
| Original language | English |
|---|---|
| Pages (from-to) | 1861-1869 |
| Number of pages | 9 |
| Journal | Nanomedicine: Nanotechnology, Biology, and Medicine |
| Volume | 11 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Oct 2015 |
| Externally published | Yes |
Keywords
- Thermo-responsive nanoparticle
- Poly(N-isopropylacrylamide)-co-acrylamide
- Retinoic acid
- Human induced pluripotent stem cells
- Neuronal differentiation
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