Thermodynamic and conformational changes of protein toward interaction with nanoparticles: a spectroscopic overview

Hojjat Alizadeh Zeinabad, Ehsan Kachooei, Ali Akbar Saboury, Irena Kostova, Farnoosh Attar, Mahsa Vaezzadeh, Mojtaba Falahati

Research output: Contribution to journalReview articleResearchpeer-review

Abstract

Nanoparticles (NPs) in different forms have been widely used in medicine and pharmaceutics for diagnosis and drug delivery. So, they are in intimate contact with bio-macromolecules like proteins which are vital and functional elements of cells and organisms. Thus, in vitro studies of the possible interaction between proteins and NPs are necessary from both application and toxicity points of view. In this article, binding characteristics and protein conformational change through interaction with various NPs have been reviewed. In recent years, a wide variety of biophysical techniques have been exploited to elucidate mechanistic aspects of NP-protein interactions. This study has focused on the information acquired from prevalent techniques including fluorescence quenching, synchronous fluorescence spectroscopy, circular dichroism (CD), and surface-enhanced Raman scattering (SERS).

LanguageEnglish
Pages105903-105919
Number of pages17
JournalRSC Advances
Volume6
Issue number107
DOIs
Publication statusPublished - 2016
Externally publishedYes

Fingerprint

Thermodynamics
Nanoparticles
Proteins
Fluorescence spectroscopy
Dichroism
Macromolecules
Drug delivery
Medicine
Toxicity
Raman scattering
Quenching
Carrier Proteins
Fluorescence
Pharmaceutical Preparations

Cite this

Zeinabad, H. A., Kachooei, E., Saboury, A. A., Kostova, I., Attar, F., Vaezzadeh, M., & Falahati, M. (2016). Thermodynamic and conformational changes of protein toward interaction with nanoparticles: a spectroscopic overview. RSC Advances, 6(107), 105903-105919. https://doi.org/10.1039/c6ra16422f
Zeinabad, Hojjat Alizadeh ; Kachooei, Ehsan ; Saboury, Ali Akbar ; Kostova, Irena ; Attar, Farnoosh ; Vaezzadeh, Mahsa ; Falahati, Mojtaba. / Thermodynamic and conformational changes of protein toward interaction with nanoparticles : a spectroscopic overview. In: RSC Advances. 2016 ; Vol. 6, No. 107. pp. 105903-105919.
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Zeinabad, HA, Kachooei, E, Saboury, AA, Kostova, I, Attar, F, Vaezzadeh, M & Falahati, M 2016, 'Thermodynamic and conformational changes of protein toward interaction with nanoparticles: a spectroscopic overview', RSC Advances, vol. 6, no. 107, pp. 105903-105919. https://doi.org/10.1039/c6ra16422f

Thermodynamic and conformational changes of protein toward interaction with nanoparticles : a spectroscopic overview. / Zeinabad, Hojjat Alizadeh; Kachooei, Ehsan; Saboury, Ali Akbar; Kostova, Irena; Attar, Farnoosh; Vaezzadeh, Mahsa; Falahati, Mojtaba.

In: RSC Advances, Vol. 6, No. 107, 2016, p. 105903-105919.

Research output: Contribution to journalReview articleResearchpeer-review

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T1 - Thermodynamic and conformational changes of protein toward interaction with nanoparticles

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AU - Zeinabad, Hojjat Alizadeh

AU - Kachooei, Ehsan

AU - Saboury, Ali Akbar

AU - Kostova, Irena

AU - Attar, Farnoosh

AU - Vaezzadeh, Mahsa

AU - Falahati, Mojtaba

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AB - Nanoparticles (NPs) in different forms have been widely used in medicine and pharmaceutics for diagnosis and drug delivery. So, they are in intimate contact with bio-macromolecules like proteins which are vital and functional elements of cells and organisms. Thus, in vitro studies of the possible interaction between proteins and NPs are necessary from both application and toxicity points of view. In this article, binding characteristics and protein conformational change through interaction with various NPs have been reviewed. In recent years, a wide variety of biophysical techniques have been exploited to elucidate mechanistic aspects of NP-protein interactions. This study has focused on the information acquired from prevalent techniques including fluorescence quenching, synchronous fluorescence spectroscopy, circular dichroism (CD), and surface-enhanced Raman scattering (SERS).

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