Thermodynamic and conformational changes of protein toward interaction with nanoparticles

a spectroscopic overview

Hojjat Alizadeh Zeinabad, Ehsan Kachooei, Ali Akbar Saboury, Irena Kostova, Farnoosh Attar, Mahsa Vaezzadeh, Mojtaba Falahati*

*Corresponding author for this work

Research output: Contribution to journalReview article

32 Citations (Scopus)

Abstract

Nanoparticles (NPs) in different forms have been widely used in medicine and pharmaceutics for diagnosis and drug delivery. So, they are in intimate contact with bio-macromolecules like proteins which are vital and functional elements of cells and organisms. Thus, in vitro studies of the possible interaction between proteins and NPs are necessary from both application and toxicity points of view. In this article, binding characteristics and protein conformational change through interaction with various NPs have been reviewed. In recent years, a wide variety of biophysical techniques have been exploited to elucidate mechanistic aspects of NP-protein interactions. This study has focused on the information acquired from prevalent techniques including fluorescence quenching, synchronous fluorescence spectroscopy, circular dichroism (CD), and surface-enhanced Raman scattering (SERS).

Original languageEnglish
Pages (from-to)105903-105919
Number of pages17
JournalRSC Advances
Volume6
Issue number107
DOIs
Publication statusPublished - 2016
Externally publishedYes

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Zeinabad, H. A., Kachooei, E., Saboury, A. A., Kostova, I., Attar, F., Vaezzadeh, M., & Falahati, M. (2016). Thermodynamic and conformational changes of protein toward interaction with nanoparticles: a spectroscopic overview. RSC Advances, 6(107), 105903-105919. https://doi.org/10.1039/c6ra16422f