Thermus diversity and strain loss during enrichment

David J. Saul*, Rosalind A. Reeves, Hugh W. Morgan, Peter L. Bergquist

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    8 Citations (Scopus)


    The diversity of Thermus strains isolated from each of two New Zealand hot pools was examined by isolating partial SSU (16S) rRNA genes and comparing their sequences. Although all of the sequences were similar, several variants were found in each pool. Standard methods for the enrichment of Thermus were then carried out and the gene isolation and sequencing procedure was performed on the enriched isolates. The enrichments resulted in the maintenance of a single dominant strain from each pool and there was a complete loss of heterogeneity in the sequences. These results demonstrate that minor differences in SSU rRNA sequence are indicative of a physiological variance between strains which is of sufficient significance to provide selective advantage or disadvantage during enrichment. Copyright (C) 1999 Federation of European Microbiological Societies.

    Original languageEnglish
    Pages (from-to)157-162
    Number of pages6
    JournalFEMS Microbiology Ecology
    Issue number2
    Publication statusPublished - Oct 1999


    • 16S
    • enrichment
    • microbial diversity
    • SSU rRNA sequences
    • thermus


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