Three-dimensional visualization of dermal skin structure using confocal laser scanning microscopy

A. P M Antunes; A. D. Covington; N. Petford; T. Murray; D. Wertheim

doi:10.1111/jmi.12052

Three-dimensional visualization of dermal skin structure using confocal laser scanning microscopy

A. P M Antunes^*, A. D. Covington, N. Petford, T. Murray, D. Wertheim

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The properties and performance of collagen-based materials may be affected by the collagen fibre bundle pattern, orientation and weave. The aim of this study was to develop and apply methods to visualize the dermis using confocal laser scanning microscopy from thin tissue sections stained with haematoxylin and eosin. The data was processed to allow three-dimensional (3-D) visualization on a PC and using a 3-D immersive technology system. The 3-D visualization of the confocal microscope image stacks allowed the evaluation of the collagen macromolecular structure including the collagen fibre bundles. The methods developed provide a novel way of viewing complex organic structures with further potential applications in the medical field.

Original language	English
Pages (from-to)	14-18
Number of pages	5
Journal	Journal of Microscopy
Volume	251
Issue number	1
DOIs	https://doi.org/10.1111/jmi.12052
Publication status	Published - Jul 2013

Keywords

Bovine
Collagen
Confocal laser scanning microscopy
Dermis
H&E
Immersive 3-D technology

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10.1111/jmi.12052

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abstract = "The properties and performance of collagen-based materials may be affected by the collagen fibre bundle pattern, orientation and weave. The aim of this study was to develop and apply methods to visualize the dermis using confocal laser scanning microscopy from thin tissue sections stained with haematoxylin and eosin. The data was processed to allow three-dimensional (3-D) visualization on a PC and using a 3-D immersive technology system. The 3-D visualization of the confocal microscope image stacks allowed the evaluation of the collagen macromolecular structure including the collagen fibre bundles. The methods developed provide a novel way of viewing complex organic structures with further potential applications in the medical field.",

keywords = "Bovine, Collagen, Confocal laser scanning microscopy, Dermis, H&E, Immersive 3-D technology",

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AU - Antunes, A. P M

AU - Covington, A. D.

AU - Petford, N.

AU - Murray, T.

AU - Wertheim, D.

PY - 2013/7

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N2 - The properties and performance of collagen-based materials may be affected by the collagen fibre bundle pattern, orientation and weave. The aim of this study was to develop and apply methods to visualize the dermis using confocal laser scanning microscopy from thin tissue sections stained with haematoxylin and eosin. The data was processed to allow three-dimensional (3-D) visualization on a PC and using a 3-D immersive technology system. The 3-D visualization of the confocal microscope image stacks allowed the evaluation of the collagen macromolecular structure including the collagen fibre bundles. The methods developed provide a novel way of viewing complex organic structures with further potential applications in the medical field.

AB - The properties and performance of collagen-based materials may be affected by the collagen fibre bundle pattern, orientation and weave. The aim of this study was to develop and apply methods to visualize the dermis using confocal laser scanning microscopy from thin tissue sections stained with haematoxylin and eosin. The data was processed to allow three-dimensional (3-D) visualization on a PC and using a 3-D immersive technology system. The 3-D visualization of the confocal microscope image stacks allowed the evaluation of the collagen macromolecular structure including the collagen fibre bundles. The methods developed provide a novel way of viewing complex organic structures with further potential applications in the medical field.

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KW - Collagen

KW - Confocal laser scanning microscopy

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KW - H&E

KW - Immersive 3-D technology

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Three-dimensional visualization of dermal skin structure using confocal laser scanning microscopy

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Keywords

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