Three new pathogenicity genes in Leptosphaeria maculans identified by Agrobacterium-mediated insertional mutagenesis

Andrew S. Urquhart; Alexander Idnurm

doi:10.1007/s13313-023-00929-5

Three new pathogenicity genes in Leptosphaeria maculans identified by Agrobacterium-mediated insertional mutagenesis

Andrew S. Urquhart, Alexander Idnurm^*

^*Corresponding author for this work

Applied BioSciences

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Abstract

Leptosphaeria maculans causes blackleg disease of Brassica napus, but the genetic basis for how this filamentous fungus damages canola plants is not well understood. Here, non-pathogenic mutants were identified from an Agrobacterium-mediated insertional mutagenic screen, and three of the mutants were complemented to indicate their involvement in pathogenicity. The genes encode a putative flavoprotein, a HEAT repeat-containing protein and a homolog of the Spt8 component of the Spt-Ada-Gcn5 acetyltransferase (SAGA) complex. The little commonality between known pathogenicity genes of this species suggests that the quest for understanding how L. maculans causes disease from a gene perspective is still at an early stage.

Original language	English
Pages (from-to)	373-376
Number of pages	4
Journal	Australasian Plant Pathology
Volume	52
Issue number	4
Early online date	19 Jun 2023
DOIs	https://doi.org/10.1007/s13313-023-00929-5
Publication status	Published - Jul 2023

Bibliographical note

Copyright the Author(s) 2023. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

Canola
Dothideomycete
Insertional mutation
Phytopathogen

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10.1007/s13313-023-00929-5

Publisher version (open access)Final published version, 1.82 MBLicence: CC BY

Cite this

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abstract = "Leptosphaeria maculans causes blackleg disease of Brassica napus, but the genetic basis for how this filamentous fungus damages canola plants is not well understood. Here, non-pathogenic mutants were identified from an Agrobacterium-mediated insertional mutagenic screen, and three of the mutants were complemented to indicate their involvement in pathogenicity. The genes encode a putative flavoprotein, a HEAT repeat-containing protein and a homolog of the Spt8 component of the Spt-Ada-Gcn5 acetyltransferase (SAGA) complex. The little commonality between known pathogenicity genes of this species suggests that the quest for understanding how L. maculans causes disease from a gene perspective is still at an early stage.",

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Three new pathogenicity genes in Leptosphaeria maculans identified by Agrobacterium-mediated insertional mutagenesis

Abstract

Bibliographical note

Keywords

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