Tiliqua rugosa microsatellites

Isolation via enrichment and characterisation of loci for multiplex PCR in T. rugosa and the endangered T. adelaidensis

Michael G. Gardner*, Juan J. Sanchez, Rachael Y. Dudaniec, Leah Rheinberger, Annabel L. Smith, Kathleen M. Saint

*Corresponding author for this work

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We used an enrichment technique to isolate 18 novel di and tri microsatellites for the socially monogamous lizard Tiliqua rugosa. These loci were amplified in conjunction with previously described loci in two and three PCR multiplexes for T. rugosa and the endangered T. adelaidensis, respectively. The loci were highly polymorphic in both species, exhibiting between 2 and 32 alleles with observed heterozygosity ranging from 0.43 to 0.96. These markers will be useful for population-level analyses and can contribute to a genetic foundation for conservation strategies for the endangered T. adelaidensis.

Original languageEnglish
Pages (from-to)233-237
Number of pages5
JournalConservation Genetics
Volume9
Issue number1
DOIs
Publication statusPublished - Feb 2008
Externally publishedYes

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