Time-gated real-time bioimaging system using multicolor microsecond-lifetime silica nanoparticles

Dayong Jin*, James Piper, Jingli Yuan, Robert Leif

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contributionpeer-review

7 Citations (Scopus)
26 Downloads (Pure)

Abstract

In advanced cytometry, a fundamental challenge for rapid specific detection of rare-event micro-organisms is the autofluorescence noise from the complex biological samples. Time-gated luminescence can effectively discriminate labeled cells from autofluorescence background. Recently, a real-time true-colour time-gated luminescence microscopy system has been developed based on the synchronization of a solid-state excitation source and a super-fast optical shutter. We also developed a variety of ultra-bright silica nano-biolabels with multiple luminescence colours and controllable lifetimes in microsecond range. These developments allowed the development of an advanced cell analysis system for real-time background-free imaging and rare-event counting of microsecond-lifetime multi-colour labelled water-borne pathogens.

Original languageEnglish
Title of host publicationImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
EditorsDaniel L. Farkas, Dan V. Nicolau, Robert C. Leif
Place of PublicationWashington, DC
PublisherSPIE
Pages756819-1-756819-12
Number of pages12
Volume7568
ISBN (Print)9780819479648
DOIs
Publication statusPublished - 2010
EventImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII - San Francisco, CA, United States
Duration: 23 Jan 201025 Jan 2010

Other

OtherImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
CountryUnited States
CitySan Francisco, CA
Period23/01/1025/01/10

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