Time-gated real-time bioimaging system using multicolor microsecond-lifetime silica nanoparticles

Dayong Jin; James Piper; Jingli Yuan; Robert Leif

doi:10.1117/12.841569

Time-gated real-time bioimaging system using multicolor microsecond-lifetime silica nanoparticles

Dayong Jin^*, James Piper, Jingli Yuan, Robert Leif

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution › peer-review

8 Citations (Scopus)

72 Downloads (Pure)

Abstract

In advanced cytometry, a fundamental challenge for rapid specific detection of rare-event micro-organisms is the autofluorescence noise from the complex biological samples. Time-gated luminescence can effectively discriminate labeled cells from autofluorescence background. Recently, a real-time true-colour time-gated luminescence microscopy system has been developed based on the synchronization of a solid-state excitation source and a super-fast optical shutter. We also developed a variety of ultra-bright silica nano-biolabels with multiple luminescence colours and controllable lifetimes in microsecond range. These developments allowed the development of an advanced cell analysis system for real-time background-free imaging and rare-event counting of microsecond-lifetime multi-colour labelled water-borne pathogens.

Original language	English
Title of host publication	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
Editors	Daniel L. Farkas, Dan V. Nicolau, Robert C. Leif
Place of Publication	Washington, DC
Publisher	SPIE
Pages	756819-1-756819-12
Number of pages	12
Volume	7568
ISBN (Print)	9780819479648
DOIs	https://doi.org/10.1117/12.841569
Publication status	Published - 2010
Event	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII - San Francisco, CA, United States Duration: 23 Jan 2010 → 25 Jan 2010

Other

Other	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
Country/Territory	United States
City	San Francisco, CA
Period	23/01/10 → 25/01/10

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title = "Time-gated real-time bioimaging system using multicolor microsecond-lifetime silica nanoparticles",

abstract = "In advanced cytometry, a fundamental challenge for rapid specific detection of rare-event micro-organisms is the autofluorescence noise from the complex biological samples. Time-gated luminescence can effectively discriminate labeled cells from autofluorescence background. Recently, a real-time true-colour time-gated luminescence microscopy system has been developed based on the synchronization of a solid-state excitation source and a super-fast optical shutter. We also developed a variety of ultra-bright silica nano-biolabels with multiple luminescence colours and controllable lifetimes in microsecond range. These developments allowed the development of an advanced cell analysis system for real-time background-free imaging and rare-event counting of microsecond-lifetime multi-colour labelled water-borne pathogens.",

author = "Dayong Jin and James Piper and Jingli Yuan and Robert Leif",

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doi = "10.1117/12.841569",

language = "English",

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booktitle = "Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII",

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note = "Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII ; Conference date: 23-01-2010 Through 25-01-2010",

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Jin, D, Piper, J, Yuan, J & Leif, R 2010, Time-gated real-time bioimaging system using multicolor microsecond-lifetime silica nanoparticles. in DL Farkas, DV Nicolau & RC Leif (eds), Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII. vol. 7568, 756819, SPIE, Washington, DC, pp. 756819-1-756819-12, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, San Francisco, CA, United States, 23/01/10. https://doi.org/10.1117/12.841569

Time-gated real-time bioimaging system using multicolor microsecond-lifetime silica nanoparticles. / Jin, Dayong; Piper, James; Yuan, Jingli et al.
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII. ed. / Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif. Vol. 7568 Washington, DC: SPIE, 2010. p. 756819-1-756819-12 756819.

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution › peer-review

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AU - Piper, James

AU - Yuan, Jingli

AU - Leif, Robert

PY - 2010

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N2 - In advanced cytometry, a fundamental challenge for rapid specific detection of rare-event micro-organisms is the autofluorescence noise from the complex biological samples. Time-gated luminescence can effectively discriminate labeled cells from autofluorescence background. Recently, a real-time true-colour time-gated luminescence microscopy system has been developed based on the synchronization of a solid-state excitation source and a super-fast optical shutter. We also developed a variety of ultra-bright silica nano-biolabels with multiple luminescence colours and controllable lifetimes in microsecond range. These developments allowed the development of an advanced cell analysis system for real-time background-free imaging and rare-event counting of microsecond-lifetime multi-colour labelled water-borne pathogens.

AB - In advanced cytometry, a fundamental challenge for rapid specific detection of rare-event micro-organisms is the autofluorescence noise from the complex biological samples. Time-gated luminescence can effectively discriminate labeled cells from autofluorescence background. Recently, a real-time true-colour time-gated luminescence microscopy system has been developed based on the synchronization of a solid-state excitation source and a super-fast optical shutter. We also developed a variety of ultra-bright silica nano-biolabels with multiple luminescence colours and controllable lifetimes in microsecond range. These developments allowed the development of an advanced cell analysis system for real-time background-free imaging and rare-event counting of microsecond-lifetime multi-colour labelled water-borne pathogens.

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SN - 9780819479648

VL - 7568

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BT - Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII

A2 - Farkas, Daniel L.

A2 - Nicolau, Dan V.

A2 - Leif, Robert C.

PB - SPIE

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T2 - Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII

Y2 - 23 January 2010 through 25 January 2010

ER -

Time-gated real-time bioimaging system using multicolor microsecond-lifetime silica nanoparticles

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