To assess the effectiveness of topical negative pressure (TNP) combined with local instillation of biocides against Staphylococcus epidermidis and Pseudomonas aeruginosa biofilm in vitro

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Abstract

Introduction: S epidermidis is a recognised major biofilm pathogen and has been implicated in medical device contamination ranging from artificial hip prostheses to breast implants. Pseudomonas aeruginosa is a known pathogen in chronic wounds. Within biofilms, bacteria are enclosed in a polymeric matrix or EPS which cements the bacteria to each other and to the surface. Biofilms act to protect the bacteria from the environment increasing resistance to host immunity, antibiotics and biocides. More recently, we have shown that biofilm occurs on the surface of chronic wounds and that this may have aetiological implications.
Method: Staph spp and pseudomonas spp biofilm were grown on glass coupons using the CDC biofilm reactor for 48 hrs and the coupons randomly inserted into the wound model and for 24 hrs subjected to:
1. No instillation plus TNP
2. 0.1% w/v formulated hypocholorous acid instillation plus TNP
3. Saline instillation (no biocide) plus TNP
4. Betadine instillation plus TNP
Betadine and saline instillations were for 30 minutes every 8, 4 and 2 hours per day in separate experiments. NVC instillation was for 3 minutes, every 8, 4 and 2 hours per day in separate experiments. Following treatment, the coupons were carefully extracted to avoid damaging the biofilms. The effect of TNP on the biofilms was then assessed by colony forming units and electron microscopy.
Results: The results show that lower frequency of instillation did not have significant effect on bacterial load for both types of bacteria. Increase in frequency of instillations resulted in no growth of pseudomonas while increase in frequency of instillations resulted in a significant decrease in staph spp.

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Staphylococcus epidermidis
Disinfectants
Biofilms
Pseudomonas aeruginosa
Pressure
Bacteria
Povidone-Iodine
Pseudomonas
Wounds and Injuries
Breast Implants
Hip Prosthesis
In Vitro Techniques
Bacterial Load
Centers for Disease Control and Prevention (U.S.)
Glass
Immunity
Electron Microscopy
Stem Cells
Anti-Bacterial Agents
Equipment and Supplies

Cite this

@article{778110ed3866443895b27889ef8198ae,
title = "To assess the effectiveness of topical negative pressure (TNP) combined with local instillation of biocides against Staphylococcus epidermidis and Pseudomonas aeruginosa biofilm in vitro",
abstract = "Introduction: S epidermidis is a recognised major biofilm pathogen and has been implicated in medical device contamination ranging from artificial hip prostheses to breast implants. Pseudomonas aeruginosa is a known pathogen in chronic wounds. Within biofilms, bacteria are enclosed in a polymeric matrix or EPS which cements the bacteria to each other and to the surface. Biofilms act to protect the bacteria from the environment increasing resistance to host immunity, antibiotics and biocides. More recently, we have shown that biofilm occurs on the surface of chronic wounds and that this may have aetiological implications. Method: Staph spp and pseudomonas spp biofilm were grown on glass coupons using the CDC biofilm reactor for 48 hrs and the coupons randomly inserted into the wound model and for 24 hrs subjected to: 1. No instillation plus TNP 2. 0.1{\%} w/v formulated hypocholorous acid instillation plus TNP 3. Saline instillation (no biocide) plus TNP 4. Betadine instillation plus TNP Betadine and saline instillations were for 30 minutes every 8, 4 and 2 hours per day in separate experiments. NVC instillation was for 3 minutes, every 8, 4 and 2 hours per day in separate experiments. Following treatment, the coupons were carefully extracted to avoid damaging the biofilms. The effect of TNP on the biofilms was then assessed by colony forming units and electron microscopy. Results: The results show that lower frequency of instillation did not have significant effect on bacterial load for both types of bacteria. Increase in frequency of instillations resulted in no growth of pseudomonas while increase in frequency of instillations resulted in a significant decrease in staph spp.",
author = "R. Mittal and K. Vickery and Q. Ngo and Deva, {A. K.}",
year = "2009",
month = "5",
doi = "10.1111/j.1445-2197.2009.04927_52.x",
language = "English",
volume = "79",
pages = "A64--A65",
journal = "ANZ journal of surgery : abstracts of the Royal Australasian College of Surgeons’ Annual Scientific Congress",
issn = "1445-1433",
publisher = "Wiley-Blackwell, Wiley",
number = "s1",

}

TY - JOUR

T1 - To assess the effectiveness of topical negative pressure (TNP) combined with local instillation of biocides against Staphylococcus epidermidis and Pseudomonas aeruginosa biofilm in vitro

AU - Mittal,R.

AU - Vickery,K.

AU - Ngo,Q.

AU - Deva,A. K.

PY - 2009/5

Y1 - 2009/5

N2 - Introduction: S epidermidis is a recognised major biofilm pathogen and has been implicated in medical device contamination ranging from artificial hip prostheses to breast implants. Pseudomonas aeruginosa is a known pathogen in chronic wounds. Within biofilms, bacteria are enclosed in a polymeric matrix or EPS which cements the bacteria to each other and to the surface. Biofilms act to protect the bacteria from the environment increasing resistance to host immunity, antibiotics and biocides. More recently, we have shown that biofilm occurs on the surface of chronic wounds and that this may have aetiological implications. Method: Staph spp and pseudomonas spp biofilm were grown on glass coupons using the CDC biofilm reactor for 48 hrs and the coupons randomly inserted into the wound model and for 24 hrs subjected to: 1. No instillation plus TNP 2. 0.1% w/v formulated hypocholorous acid instillation plus TNP 3. Saline instillation (no biocide) plus TNP 4. Betadine instillation plus TNP Betadine and saline instillations were for 30 minutes every 8, 4 and 2 hours per day in separate experiments. NVC instillation was for 3 minutes, every 8, 4 and 2 hours per day in separate experiments. Following treatment, the coupons were carefully extracted to avoid damaging the biofilms. The effect of TNP on the biofilms was then assessed by colony forming units and electron microscopy. Results: The results show that lower frequency of instillation did not have significant effect on bacterial load for both types of bacteria. Increase in frequency of instillations resulted in no growth of pseudomonas while increase in frequency of instillations resulted in a significant decrease in staph spp.

AB - Introduction: S epidermidis is a recognised major biofilm pathogen and has been implicated in medical device contamination ranging from artificial hip prostheses to breast implants. Pseudomonas aeruginosa is a known pathogen in chronic wounds. Within biofilms, bacteria are enclosed in a polymeric matrix or EPS which cements the bacteria to each other and to the surface. Biofilms act to protect the bacteria from the environment increasing resistance to host immunity, antibiotics and biocides. More recently, we have shown that biofilm occurs on the surface of chronic wounds and that this may have aetiological implications. Method: Staph spp and pseudomonas spp biofilm were grown on glass coupons using the CDC biofilm reactor for 48 hrs and the coupons randomly inserted into the wound model and for 24 hrs subjected to: 1. No instillation plus TNP 2. 0.1% w/v formulated hypocholorous acid instillation plus TNP 3. Saline instillation (no biocide) plus TNP 4. Betadine instillation plus TNP Betadine and saline instillations were for 30 minutes every 8, 4 and 2 hours per day in separate experiments. NVC instillation was for 3 minutes, every 8, 4 and 2 hours per day in separate experiments. Following treatment, the coupons were carefully extracted to avoid damaging the biofilms. The effect of TNP on the biofilms was then assessed by colony forming units and electron microscopy. Results: The results show that lower frequency of instillation did not have significant effect on bacterial load for both types of bacteria. Increase in frequency of instillations resulted in no growth of pseudomonas while increase in frequency of instillations resulted in a significant decrease in staph spp.

U2 - 10.1111/j.1445-2197.2009.04927_52.x

DO - 10.1111/j.1445-2197.2009.04927_52.x

M3 - Meeting abstract

VL - 79

SP - A64-A65

JO - ANZ journal of surgery : abstracts of the Royal Australasian College of Surgeons’ Annual Scientific Congress

T2 - ANZ journal of surgery : abstracts of the Royal Australasian College of Surgeons’ Annual Scientific Congress

JF - ANZ journal of surgery : abstracts of the Royal Australasian College of Surgeons’ Annual Scientific Congress

SN - 1445-1433

IS - s1

M1 - PR52P

ER -