Tonometric assessment of jejunal mucosal nitric oxide formation in anaesthetized pigs

Johan Snygg*, A. Casselbrant, A. Pettersson, M. Holm, L. Fändriks, A. Åneman

*Corresponding author for this work

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Nitric oxide (NO) in the gut has attracted increasing interest as a regulatory factor for a wide variety of intestinal functions. This study was performed to evaluate some methodological aspects and jejunal sources for NO synthesis. Bench side evaluations and an animal model using chloralose-anaesthetized pigs were used. Immunohistochemistry was performed on samples from pig intestine and direct measurements of intestinal NO formation were performed using intraluminal tonometry. Tonometric measurements were quantitatively accurate and with high reproducibility. A substantial NO formation was assessed which was markedly inhibited by luminal administration of the nonselective NOS inhibitor L-NAME. Intravenous administration of L-NAME also reduced jejunal NO formation but to a lesser extent. Immunohistochemistry revealed staining for the inducible type of NOS in the mucosal surface epithelium whereas endothelial and neuronal subtypes were located in deeper layers of the jejunal wall. The study argues for that the source of jejunal NO production, as measured by intraluminal tonometry, is located in close proximity with the intestinal mucosa. The NOS in this compartment is predominantly of the inducible type.

Original languageEnglish
Pages (from-to)39-45
Number of pages7
JournalActa Physiologica Scandinavica
Volume169
Issue number1
DOIs
Publication statusPublished - 2000

Keywords

  • Animal
  • Epithelium
  • Immunohistochemistry
  • Inducible
  • Jejunal
  • Measurement
  • Nitric oxide
  • Nitric oxide synthase

Fingerprint Dive into the research topics of 'Tonometric assessment of jejunal mucosal nitric oxide formation in anaesthetized pigs'. Together they form a unique fingerprint.

  • Cite this

    Snygg, J., Casselbrant, A., Pettersson, A., Holm, M., Fändriks, L., & Åneman, A. (2000). Tonometric assessment of jejunal mucosal nitric oxide formation in anaesthetized pigs. Acta Physiologica Scandinavica, 169(1), 39-45. https://doi.org/10.1046/j.1365-201X.2000.00678.x