Towards photodynamic therapy with ionizing radiation: nanoparticle-mediated singlet oxygen generation

Sandhya Clement, Wei Deng, Elizabeth Camilleri, Brian Wilson, Ewa Goldys

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contributionResearch

Abstract

Photodynamic therapy (PDT) is a clinically approved method for the treatment of cancer by using singlet oxygen, a highly reactive oxygen generated from a photosensitizer drug upon photoactivation. Limited light penetration depth into to the tissue means that PDT is unsuitable for deep tissue cancer treatments. This can be overcome by using X-ray /gamma rays activated nanoparticles able to trigger the photosensitizer drug and generate singlet oxygen. Additionally, inorganic nanoparticles interact more strongly with X and/or gamma rays than the tissue, allowing to concentrate the effects of radiation near nanoparticle surface and they can also be molecularly targeted to cancer cells. In this work we synthesized and characterized CeF3 nanoparticles, a well-known scintillator material. The nanoparticles were conjugated with Verteporfin, a photosensitizer drug by electrostatic interaction. We assessed the performance of CeF3 and the conjugates to generate singlet oxygen exposed to X-ray radiation. The X-ray singlet oxygen quantum yield of the nanoparticle-photosensitizer system was accurately quantified for the first time. This provided realistic estimates of the singlet oxygen dose taking into consideration the dose partition of the radiation between CeF3 and the tissue. Furthermore, we investigated gold nanoparticle-photosensitizer systems. We confirmed that pure gold nanoparticles itself generate singlet oxygen which is attributed to plasmonic effects. We found enhanced singlet oxygen generation from gold-Rose Bengal conjugates and gold nanorod–verteporfin conjugates. These singlet-oxygen-generating nanomaterials add a new dimension to radiation-assisted PDT.
LanguageEnglish
Title of host publicationColloidal nanoparticles for biomedical applications XI
EditorsWolfgang J. Parak, Marek Osinski, Xing-Jie Liang
Place of PublicationBellingham, Washington
PublisherSPIE
Pages972217-1-972217-1
Number of pages1
ISBN (Print)9781628419566
DOIs
Publication statusPublished - 2016
EventColloidal Nanoparticles for Biomedical Applications XI (2016) - San Francisco, California, United States
Duration: 13 Feb 201615 Feb 2016

Publication series

NameProceedings of SPIE
PublisherSPIE
Volume9722
ISSN (Print)1605-7422

Conference

ConferenceColloidal Nanoparticles for Biomedical Applications XI (2016)
CitySan Francisco, California, United States
Period13/02/1615/02/16

Cite this

Clement, S., Deng, W., Camilleri, E., Wilson, B., & Goldys, E. (2016). Towards photodynamic therapy with ionizing radiation: nanoparticle-mediated singlet oxygen generation. In W. J. Parak, M. Osinski, & X-J. Liang (Eds.), Colloidal nanoparticles for biomedical applications XI (pp. 972217-1-972217-1). (Proceedings of SPIE; Vol. 9722). Bellingham, Washington: SPIE. https://doi.org/10.1117/12.2209206
Clement, Sandhya ; Deng, Wei ; Camilleri, Elizabeth ; Wilson, Brian ; Goldys, Ewa. / Towards photodynamic therapy with ionizing radiation : nanoparticle-mediated singlet oxygen generation. Colloidal nanoparticles for biomedical applications XI. editor / Wolfgang J. Parak ; Marek Osinski ; Xing-Jie Liang. Bellingham, Washington : SPIE, 2016. pp. 972217-1-972217-1 (Proceedings of SPIE).
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title = "Towards photodynamic therapy with ionizing radiation: nanoparticle-mediated singlet oxygen generation",
abstract = "Photodynamic therapy (PDT) is a clinically approved method for the treatment of cancer by using singlet oxygen, a highly reactive oxygen generated from a photosensitizer drug upon photoactivation. Limited light penetration depth into to the tissue means that PDT is unsuitable for deep tissue cancer treatments. This can be overcome by using X-ray /gamma rays activated nanoparticles able to trigger the photosensitizer drug and generate singlet oxygen. Additionally, inorganic nanoparticles interact more strongly with X and/or gamma rays than the tissue, allowing to concentrate the effects of radiation near nanoparticle surface and they can also be molecularly targeted to cancer cells. In this work we synthesized and characterized CeF3 nanoparticles, a well-known scintillator material. The nanoparticles were conjugated with Verteporfin, a photosensitizer drug by electrostatic interaction. We assessed the performance of CeF3 and the conjugates to generate singlet oxygen exposed to X-ray radiation. The X-ray singlet oxygen quantum yield of the nanoparticle-photosensitizer system was accurately quantified for the first time. This provided realistic estimates of the singlet oxygen dose taking into consideration the dose partition of the radiation between CeF3 and the tissue. Furthermore, we investigated gold nanoparticle-photosensitizer systems. We confirmed that pure gold nanoparticles itself generate singlet oxygen which is attributed to plasmonic effects. We found enhanced singlet oxygen generation from gold-Rose Bengal conjugates and gold nanorod–verteporfin conjugates. These singlet-oxygen-generating nanomaterials add a new dimension to radiation-assisted PDT.",
author = "Sandhya Clement and Wei Deng and Elizabeth Camilleri and Brian Wilson and Ewa Goldys",
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Clement, S, Deng, W, Camilleri, E, Wilson, B & Goldys, E 2016, Towards photodynamic therapy with ionizing radiation: nanoparticle-mediated singlet oxygen generation. in WJ Parak, M Osinski & X-J Liang (eds), Colloidal nanoparticles for biomedical applications XI. Proceedings of SPIE, vol. 9722, SPIE, Bellingham, Washington, pp. 972217-1-972217-1, Colloidal Nanoparticles for Biomedical Applications XI (2016), San Francisco, California, United States, 13/02/16. https://doi.org/10.1117/12.2209206

Towards photodynamic therapy with ionizing radiation : nanoparticle-mediated singlet oxygen generation. / Clement, Sandhya; Deng, Wei; Camilleri, Elizabeth; Wilson, Brian; Goldys, Ewa.

Colloidal nanoparticles for biomedical applications XI. ed. / Wolfgang J. Parak; Marek Osinski; Xing-Jie Liang. Bellingham, Washington : SPIE, 2016. p. 972217-1-972217-1 (Proceedings of SPIE; Vol. 9722).

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contributionResearch

TY - GEN

T1 - Towards photodynamic therapy with ionizing radiation

T2 - nanoparticle-mediated singlet oxygen generation

AU - Clement, Sandhya

AU - Deng, Wei

AU - Camilleri, Elizabeth

AU - Wilson, Brian

AU - Goldys, Ewa

PY - 2016

Y1 - 2016

N2 - Photodynamic therapy (PDT) is a clinically approved method for the treatment of cancer by using singlet oxygen, a highly reactive oxygen generated from a photosensitizer drug upon photoactivation. Limited light penetration depth into to the tissue means that PDT is unsuitable for deep tissue cancer treatments. This can be overcome by using X-ray /gamma rays activated nanoparticles able to trigger the photosensitizer drug and generate singlet oxygen. Additionally, inorganic nanoparticles interact more strongly with X and/or gamma rays than the tissue, allowing to concentrate the effects of radiation near nanoparticle surface and they can also be molecularly targeted to cancer cells. In this work we synthesized and characterized CeF3 nanoparticles, a well-known scintillator material. The nanoparticles were conjugated with Verteporfin, a photosensitizer drug by electrostatic interaction. We assessed the performance of CeF3 and the conjugates to generate singlet oxygen exposed to X-ray radiation. The X-ray singlet oxygen quantum yield of the nanoparticle-photosensitizer system was accurately quantified for the first time. This provided realistic estimates of the singlet oxygen dose taking into consideration the dose partition of the radiation between CeF3 and the tissue. Furthermore, we investigated gold nanoparticle-photosensitizer systems. We confirmed that pure gold nanoparticles itself generate singlet oxygen which is attributed to plasmonic effects. We found enhanced singlet oxygen generation from gold-Rose Bengal conjugates and gold nanorod–verteporfin conjugates. These singlet-oxygen-generating nanomaterials add a new dimension to radiation-assisted PDT.

AB - Photodynamic therapy (PDT) is a clinically approved method for the treatment of cancer by using singlet oxygen, a highly reactive oxygen generated from a photosensitizer drug upon photoactivation. Limited light penetration depth into to the tissue means that PDT is unsuitable for deep tissue cancer treatments. This can be overcome by using X-ray /gamma rays activated nanoparticles able to trigger the photosensitizer drug and generate singlet oxygen. Additionally, inorganic nanoparticles interact more strongly with X and/or gamma rays than the tissue, allowing to concentrate the effects of radiation near nanoparticle surface and they can also be molecularly targeted to cancer cells. In this work we synthesized and characterized CeF3 nanoparticles, a well-known scintillator material. The nanoparticles were conjugated with Verteporfin, a photosensitizer drug by electrostatic interaction. We assessed the performance of CeF3 and the conjugates to generate singlet oxygen exposed to X-ray radiation. The X-ray singlet oxygen quantum yield of the nanoparticle-photosensitizer system was accurately quantified for the first time. This provided realistic estimates of the singlet oxygen dose taking into consideration the dose partition of the radiation between CeF3 and the tissue. Furthermore, we investigated gold nanoparticle-photosensitizer systems. We confirmed that pure gold nanoparticles itself generate singlet oxygen which is attributed to plasmonic effects. We found enhanced singlet oxygen generation from gold-Rose Bengal conjugates and gold nanorod–verteporfin conjugates. These singlet-oxygen-generating nanomaterials add a new dimension to radiation-assisted PDT.

U2 - 10.1117/12.2209206

DO - 10.1117/12.2209206

M3 - Conference proceeding contribution

SN - 9781628419566

T3 - Proceedings of SPIE

SP - 972217-1-972217-1

BT - Colloidal nanoparticles for biomedical applications XI

A2 - Parak, Wolfgang J.

A2 - Osinski, Marek

A2 - Liang, Xing-Jie

PB - SPIE

CY - Bellingham, Washington

ER -

Clement S, Deng W, Camilleri E, Wilson B, Goldys E. Towards photodynamic therapy with ionizing radiation: nanoparticle-mediated singlet oxygen generation. In Parak WJ, Osinski M, Liang X-J, editors, Colloidal nanoparticles for biomedical applications XI. Bellingham, Washington: SPIE. 2016. p. 972217-1-972217-1. (Proceedings of SPIE). https://doi.org/10.1117/12.2209206