Tracking and characterizing the head motion of unanaesthetized rats in positron emission tomography

Andre Kyme, Steven Meikle, Clive Baldock, Roger Fulton

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Positron emission tomography (PET) is an important in vivo molecular imaging technique for translational research. Imaging unanaesthetized rats using motion-compensated PET avoids the confounding impact of anaesthetic drugs and enables animals to be imaged during normal or evoked behaviour. However, there is little published data on the nature of rat head motion to inform the design of suitable marker-based motion-tracking set-ups for brain imaging—specifically, set-ups that afford close to uninterrupted tracking. We performed a systematic study of rat head motion parameters for unanaesthetized tube-bound and freely moving rats with a view to designing suitable motion-tracking set-ups in each case. For tube-bound rats, using a single appropriately placed binocular tracker, uninterrupted tracking was possible greater than 95 per cent of the time. For freely moving rats, simulations and measurements of a live subject indicated that two opposed binocular trackers are sufficient (less than 10% interruption to tracking) for a wide variety of behaviour types. We conclude that reliable tracking of head pose can be achieved with marker-based optical-motion-tracking systems for both tube-bound and freely moving rats undergoing PET studies without sedation.
LanguageEnglish
Pages3094-3107
Number of pages14
JournalJournal of the Royal Society Interface
Volume9
Issue number76
DOIs
Publication statusPublished - 2012
Externally publishedYes

Fingerprint

Positron emission tomography
Positron-Emission Tomography
Rats
Head
Binoculars
Imaging techniques
Molecular imaging
Anesthetics
Molecular Imaging
Translational Medical Research
Neuroimaging
Brain
Animals

Keywords

  • rat head motion
  • motion tracking
  • brain imaging
  • positron emission tomography

Cite this

Kyme, Andre ; Meikle, Steven ; Baldock, Clive ; Fulton, Roger. / Tracking and characterizing the head motion of unanaesthetized rats in positron emission tomography. In: Journal of the Royal Society Interface. 2012 ; Vol. 9, No. 76. pp. 3094-3107.
@article{5c9fdd368a414ea59b5d3caf0b843a06,
title = "Tracking and characterizing the head motion of unanaesthetized rats in positron emission tomography",
abstract = "Positron emission tomography (PET) is an important in vivo molecular imaging technique for translational research. Imaging unanaesthetized rats using motion-compensated PET avoids the confounding impact of anaesthetic drugs and enables animals to be imaged during normal or evoked behaviour. However, there is little published data on the nature of rat head motion to inform the design of suitable marker-based motion-tracking set-ups for brain imaging—specifically, set-ups that afford close to uninterrupted tracking. We performed a systematic study of rat head motion parameters for unanaesthetized tube-bound and freely moving rats with a view to designing suitable motion-tracking set-ups in each case. For tube-bound rats, using a single appropriately placed binocular tracker, uninterrupted tracking was possible greater than 95 per cent of the time. For freely moving rats, simulations and measurements of a live subject indicated that two opposed binocular trackers are sufficient (less than 10{\%} interruption to tracking) for a wide variety of behaviour types. We conclude that reliable tracking of head pose can be achieved with marker-based optical-motion-tracking systems for both tube-bound and freely moving rats undergoing PET studies without sedation.",
keywords = "rat head motion, motion tracking, brain imaging, positron emission tomography",
author = "Andre Kyme and Steven Meikle and Clive Baldock and Roger Fulton",
year = "2012",
doi = "10.1098/​rsif.2012.0334",
language = "English",
volume = "9",
pages = "3094--3107",
journal = "Journal of the Royal Society Interface",
issn = "1742-5689",
publisher = "The Royal Society Publishing",
number = "76",

}

Tracking and characterizing the head motion of unanaesthetized rats in positron emission tomography. / Kyme, Andre; Meikle, Steven; Baldock, Clive; Fulton, Roger.

In: Journal of the Royal Society Interface, Vol. 9, No. 76, 2012, p. 3094-3107.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Tracking and characterizing the head motion of unanaesthetized rats in positron emission tomography

AU - Kyme, Andre

AU - Meikle, Steven

AU - Baldock, Clive

AU - Fulton, Roger

PY - 2012

Y1 - 2012

N2 - Positron emission tomography (PET) is an important in vivo molecular imaging technique for translational research. Imaging unanaesthetized rats using motion-compensated PET avoids the confounding impact of anaesthetic drugs and enables animals to be imaged during normal or evoked behaviour. However, there is little published data on the nature of rat head motion to inform the design of suitable marker-based motion-tracking set-ups for brain imaging—specifically, set-ups that afford close to uninterrupted tracking. We performed a systematic study of rat head motion parameters for unanaesthetized tube-bound and freely moving rats with a view to designing suitable motion-tracking set-ups in each case. For tube-bound rats, using a single appropriately placed binocular tracker, uninterrupted tracking was possible greater than 95 per cent of the time. For freely moving rats, simulations and measurements of a live subject indicated that two opposed binocular trackers are sufficient (less than 10% interruption to tracking) for a wide variety of behaviour types. We conclude that reliable tracking of head pose can be achieved with marker-based optical-motion-tracking systems for both tube-bound and freely moving rats undergoing PET studies without sedation.

AB - Positron emission tomography (PET) is an important in vivo molecular imaging technique for translational research. Imaging unanaesthetized rats using motion-compensated PET avoids the confounding impact of anaesthetic drugs and enables animals to be imaged during normal or evoked behaviour. However, there is little published data on the nature of rat head motion to inform the design of suitable marker-based motion-tracking set-ups for brain imaging—specifically, set-ups that afford close to uninterrupted tracking. We performed a systematic study of rat head motion parameters for unanaesthetized tube-bound and freely moving rats with a view to designing suitable motion-tracking set-ups in each case. For tube-bound rats, using a single appropriately placed binocular tracker, uninterrupted tracking was possible greater than 95 per cent of the time. For freely moving rats, simulations and measurements of a live subject indicated that two opposed binocular trackers are sufficient (less than 10% interruption to tracking) for a wide variety of behaviour types. We conclude that reliable tracking of head pose can be achieved with marker-based optical-motion-tracking systems for both tube-bound and freely moving rats undergoing PET studies without sedation.

KW - rat head motion

KW - motion tracking

KW - brain imaging

KW - positron emission tomography

U2 - 10.1098/​rsif.2012.0334

DO - 10.1098/​rsif.2012.0334

M3 - Article

VL - 9

SP - 3094

EP - 3107

JO - Journal of the Royal Society Interface

T2 - Journal of the Royal Society Interface

JF - Journal of the Royal Society Interface

SN - 1742-5689

IS - 76

ER -