Tracking of fast moving neuronal vesicles with ageladine A

Ulf Bickmeyer, Martin Heine, Imke Podbielski, Dennis Münd, Matthias Köck, Peter Karuso

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Ageladine A is a marine natural product that can be used to fluorescently stain living tissues and cells. Its fluorescence is highly pH dependent with the highest intensities under acidic conditions. We have used ageladine A to stain acidic vesicles in cells and found the compound especially useful for tracking transport vesicles in cultured nerve cells. Inward as well as outward ionic currents appear not to be influenced by ageladine A at concentrations of 10 μM or less. Higher concentrations than 30 μM reduce whole cell voltage dependent outward currents whereas inward currents remain unchanged up to 100 μM ageladine A (PC12 cells). Incubation with ageladine A (10 μM) in cultured hippocampal neurons does not alter miniature excitatory postsynaptic currents (mEPCS) amplitudes, frequency, rise or decay times. Fast moving vesicles are stained the brightest, suggesting they are the most acidic and likely to be Golgi derived and endocytotic vesicles for the fast anterograde and retrograde transport of proteins and other compounds needing an acidic environment.

LanguageEnglish
Pages489-494
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume402
Issue number3
DOIs
Publication statusPublished - 19 Nov 2010

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Neurons
Coloring Agents
Transport Vesicles
Excitatory Postsynaptic Potentials
PC12 Cells
Biological Products
Cultured Cells
Carrier Proteins
Fluorescence
Ageladine A
Electric potential

Cite this

Bickmeyer, Ulf ; Heine, Martin ; Podbielski, Imke ; Münd, Dennis ; Köck, Matthias ; Karuso, Peter. / Tracking of fast moving neuronal vesicles with ageladine A. In: Biochemical and Biophysical Research Communications. 2010 ; Vol. 402, No. 3. pp. 489-494.
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Tracking of fast moving neuronal vesicles with ageladine A. / Bickmeyer, Ulf; Heine, Martin; Podbielski, Imke; Münd, Dennis; Köck, Matthias; Karuso, Peter.

In: Biochemical and Biophysical Research Communications, Vol. 402, No. 3, 19.11.2010, p. 489-494.

Research output: Contribution to journalArticleResearchpeer-review

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