Transcriptome analysis reveals pathogenicity and evolutionary history of the pathogenic oomycete Pythium insidiosum

Theerapong Krajaejun*, Tassanee Lerksuthirat, Gagan Garg, Tassanee Lowhnoo, Wanta Yingyong, Rommanee Khositnithikul, Sithichoke Tangphatsornruang, Prapat Suriyaphol, Shoba Ranganathan, Thomas D. Sullivan

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

Oomycetes form a unique group of microorganisms that share hyphal morphology with fungi. Most of pathogenic oomycetes infect plants, while some species are capable of infecting animals. Pythium insidiosum is the only oomycete that can infect both humans and animals, and causes a life-threatening infectious disease, called 'pythiosis'. Controlling an infection caused by P. insidiosum is problematic because effective antimicrobial drugs are not available. Information on the biology and pathogenesis of P. insidiosum is limited. We generated a P. insidiosum transcriptome of 26735 unigenes, using the 454 sequencing platform. As adaptations to increased temperature inside human hosts are required for a successful pathogen, we generated P. insidiosum transcriptomes at 28°C and 37°C and identified 625 up-regulated and 449 down-regulated genes at 37°C. Comparing the proteomes of oomycetes, fungi, and parasites provided clues on the evolutionary history of P. insidiosum. Potential virulence factors of P. insidiosum, including putative effectors, were identified. Pythium insidiosum harbored an extensive repertoire of ~300 elicitin domain-containing proteins. The transcriptome, presented herein, provides an invaluable resource for exploring P. insidiosum's biology, pathogenesis, and evolution.

Original languageEnglish
Pages (from-to)640-653
Number of pages14
JournalFungal Biology
Volume118
Issue number7
DOIs
Publication statusPublished - Jul 2014

Keywords

  • Effector
  • Expressed sequence tag
  • Human pathogen
  • Pythiosis
  • Virulence factor

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