TY - JOUR
T1 - Transcriptome analysis reveals pathogenicity and evolutionary history of the pathogenic oomycete Pythium insidiosum
AU - Krajaejun, Theerapong
AU - Lerksuthirat, Tassanee
AU - Garg, Gagan
AU - Lowhnoo, Tassanee
AU - Yingyong, Wanta
AU - Khositnithikul, Rommanee
AU - Tangphatsornruang, Sithichoke
AU - Suriyaphol, Prapat
AU - Ranganathan, Shoba
AU - Sullivan, Thomas D.
PY - 2014/7
Y1 - 2014/7
N2 - Oomycetes form a unique group of microorganisms that share hyphal morphology with fungi. Most of pathogenic oomycetes infect plants, while some species are capable of infecting animals. Pythium insidiosum is the only oomycete that can infect both humans and animals, and causes a life-threatening infectious disease, called 'pythiosis'. Controlling an infection caused by P. insidiosum is problematic because effective antimicrobial drugs are not available. Information on the biology and pathogenesis of P. insidiosum is limited. We generated a P. insidiosum transcriptome of 26735 unigenes, using the 454 sequencing platform. As adaptations to increased temperature inside human hosts are required for a successful pathogen, we generated P. insidiosum transcriptomes at 28°C and 37°C and identified 625 up-regulated and 449 down-regulated genes at 37°C. Comparing the proteomes of oomycetes, fungi, and parasites provided clues on the evolutionary history of P. insidiosum. Potential virulence factors of P. insidiosum, including putative effectors, were identified. Pythium insidiosum harbored an extensive repertoire of ~300 elicitin domain-containing proteins. The transcriptome, presented herein, provides an invaluable resource for exploring P. insidiosum's biology, pathogenesis, and evolution.
AB - Oomycetes form a unique group of microorganisms that share hyphal morphology with fungi. Most of pathogenic oomycetes infect plants, while some species are capable of infecting animals. Pythium insidiosum is the only oomycete that can infect both humans and animals, and causes a life-threatening infectious disease, called 'pythiosis'. Controlling an infection caused by P. insidiosum is problematic because effective antimicrobial drugs are not available. Information on the biology and pathogenesis of P. insidiosum is limited. We generated a P. insidiosum transcriptome of 26735 unigenes, using the 454 sequencing platform. As adaptations to increased temperature inside human hosts are required for a successful pathogen, we generated P. insidiosum transcriptomes at 28°C and 37°C and identified 625 up-regulated and 449 down-regulated genes at 37°C. Comparing the proteomes of oomycetes, fungi, and parasites provided clues on the evolutionary history of P. insidiosum. Potential virulence factors of P. insidiosum, including putative effectors, were identified. Pythium insidiosum harbored an extensive repertoire of ~300 elicitin domain-containing proteins. The transcriptome, presented herein, provides an invaluable resource for exploring P. insidiosum's biology, pathogenesis, and evolution.
KW - Effector
KW - Expressed sequence tag
KW - Human pathogen
KW - Pythiosis
KW - Virulence factor
UR - http://www.scopus.com/inward/record.url?scp=84905216938&partnerID=8YFLogxK
U2 - 10.1016/j.funbio.2014.01.009
DO - 10.1016/j.funbio.2014.01.009
M3 - Article
C2 - 25088078
AN - SCOPUS:84905216938
SN - 1878-6146
VL - 118
SP - 640
EP - 653
JO - Fungal Biology
JF - Fungal Biology
IS - 7
ER -