Transcriptomic changes in hot spring frog tadpoles (Buergeria otai) in response to heat stress

Shohei Komaki; Masatoshi Matsunami; Jhan-Wei Lin; Ko-Huan Lee; Yen-Po Lin; Yu Lee; Si-Min Lin; Takeshi Igawa

doi:10.3389/fevo.2021.706887

Transcriptomic changes in hot spring frog tadpoles (Buergeria otai) in response to heat stress

Shohei Komaki, Masatoshi Matsunami, Jhan-Wei Lin, Ko-Huan Lee, Yen-Po Lin, Yu Lee, Si-Min Lin, Takeshi Igawa^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

Buergeria frog tadpoles exhibit high thermal tolerance and are occasionally found in water pools that temporarily exceed 40°C. With the aim of understanding how they can cope with the severe heat stress, we performed RNA-seq of three heat-treated (38°C) and three control (25°C) tadpoles and compared their transcriptomic profiles. We identified 382 differentially expressed transcripts. A protein-protein interaction (PPI) network analysis of these transcripts further identified hub proteins involved in protein degradation, stress granule assembly, and global suppression of DNA transcription and mRNA translation. Along with the avoidance behavior against high water temperature, these endurance mechanisms potentially support tadpoles to survive in high temperatures for short periods of time. Similar mechanisms may exist in many other amphibian species whose habitats are prone to high temperatures.

Original language	English
Article number	706887
Pages (from-to)	1-9
Number of pages	9
Journal	Frontiers in Ecology and Evolution
Volume	9
DOIs	https://doi.org/10.3389/fevo.2021.706887
Publication status	Published - 13 Oct 2021

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Copyright the Author(s) 2021. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

amphibia
anura
differentially expressed genes
protein-protein interaction network
RNA-seq
high thermal tolerance
heat stress response

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10.3389/fevo.2021.706887

Publisher version (open access)Final published version, 487 KBLicence: CC BY

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title = "Transcriptomic changes in hot spring frog tadpoles (Buergeria otai) in response to heat stress",

abstract = "Buergeria frog tadpoles exhibit high thermal tolerance and are occasionally found in water pools that temporarily exceed 40°C. With the aim of understanding how they can cope with the severe heat stress, we performed RNA-seq of three heat-treated (38°C) and three control (25°C) tadpoles and compared their transcriptomic profiles. We identified 382 differentially expressed transcripts. A protein-protein interaction (PPI) network analysis of these transcripts further identified hub proteins involved in protein degradation, stress granule assembly, and global suppression of DNA transcription and mRNA translation. Along with the avoidance behavior against high water temperature, these endurance mechanisms potentially support tadpoles to survive in high temperatures for short periods of time. Similar mechanisms may exist in many other amphibian species whose habitats are prone to high temperatures.",

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author = "Shohei Komaki and Masatoshi Matsunami and Jhan-Wei Lin and Ko-Huan Lee and Yen-Po Lin and Yu Lee and Si-Min Lin and Takeshi Igawa",

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doi = "10.3389/fevo.2021.706887",

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AU - Komaki, Shohei

AU - Matsunami, Masatoshi

AU - Lin, Jhan-Wei

AU - Lee, Ko-Huan

AU - Lin, Yen-Po

AU - Lee, Yu

AU - Lin, Si-Min

AU - Igawa, Takeshi

N1 - Copyright the Author(s) 2021. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

PY - 2021/10/13

Y1 - 2021/10/13

N2 - Buergeria frog tadpoles exhibit high thermal tolerance and are occasionally found in water pools that temporarily exceed 40°C. With the aim of understanding how they can cope with the severe heat stress, we performed RNA-seq of three heat-treated (38°C) and three control (25°C) tadpoles and compared their transcriptomic profiles. We identified 382 differentially expressed transcripts. A protein-protein interaction (PPI) network analysis of these transcripts further identified hub proteins involved in protein degradation, stress granule assembly, and global suppression of DNA transcription and mRNA translation. Along with the avoidance behavior against high water temperature, these endurance mechanisms potentially support tadpoles to survive in high temperatures for short periods of time. Similar mechanisms may exist in many other amphibian species whose habitats are prone to high temperatures.

AB - Buergeria frog tadpoles exhibit high thermal tolerance and are occasionally found in water pools that temporarily exceed 40°C. With the aim of understanding how they can cope with the severe heat stress, we performed RNA-seq of three heat-treated (38°C) and three control (25°C) tadpoles and compared their transcriptomic profiles. We identified 382 differentially expressed transcripts. A protein-protein interaction (PPI) network analysis of these transcripts further identified hub proteins involved in protein degradation, stress granule assembly, and global suppression of DNA transcription and mRNA translation. Along with the avoidance behavior against high water temperature, these endurance mechanisms potentially support tadpoles to survive in high temperatures for short periods of time. Similar mechanisms may exist in many other amphibian species whose habitats are prone to high temperatures.

KW - amphibia

KW - anura

KW - differentially expressed genes

KW - protein-protein interaction network

KW - RNA-seq

KW - high thermal tolerance

KW - heat stress response

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DO - 10.3389/fevo.2021.706887

M3 - Article

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SN - 2296-701X

VL - 9

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JO - Frontiers in Ecology and Evolution

JF - Frontiers in Ecology and Evolution

M1 - 706887

ER -

Transcriptomic changes in hot spring frog tadpoles (Buergeria otai) in response to heat stress

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