Transfection and imaging of diamond nanocrystals as scattering optical labels

Bradley R. Smith; Marcus Niebert; Taras Plakhotnik; Andrei V. Zvyagin

doi:10.1016/j.jlumin.2007.02.044

Transfection and imaging of diamond nanocrystals as scattering optical labels

Bradley R. Smith^*, Marcus Niebert, Taras Plakhotnik, Andrei V. Zvyagin

^*Corresponding author for this work

Research output: Contribution to journal › Article

41 Citations (Scopus)

Abstract

We report on the first demonstration of nanodiamond (ND) as a scattering optical label in a biological environment. NDs were efficiently transfected into cells using cationic liposomes, and imaged using differential interference and Hoffman modulation 'space' contrast microscopy techniques. We have shown that 55 nm NDs are biologically inert and produce a bright signal compared to the cell background. ND as a scattering label presents the possibility for extended biological imaging with relatively little thermal or biochemical perturbations due to the optical transparency and biologically inert nature of diamond.

Original language	English
Pages (from-to)	260-263
Number of pages	4
Journal	Journal of Luminescence
Volume	127
Issue number	1
DOIs	https://doi.org/10.1016/j.jlumin.2007.02.044
Publication status	Published - Nov 2007
Externally published	Yes

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Smith, B. R., Niebert, M., Plakhotnik, T., & Zvyagin, A. V. (2007). Transfection and imaging of diamond nanocrystals as scattering optical labels. Journal of Luminescence, 127(1), 260-263. https://doi.org/10.1016/j.jlumin.2007.02.044

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AB - We report on the first demonstration of nanodiamond (ND) as a scattering optical label in a biological environment. NDs were efficiently transfected into cells using cationic liposomes, and imaged using differential interference and Hoffman modulation 'space' contrast microscopy techniques. We have shown that 55 nm NDs are biologically inert and produce a bright signal compared to the cell background. ND as a scattering label presents the possibility for extended biological imaging with relatively little thermal or biochemical perturbations due to the optical transparency and biologically inert nature of diamond.

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10.1016/j.jlumin.2007.02.044

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