Transfection and imaging of diamond nanocrystals as scattering optical labels

Bradley R. Smith; Marcus Niebert; Taras Plakhotnik; Andrei V. Zvyagin

doi:10.1016/j.jlumin.2007.02.044

Transfection and imaging of diamond nanocrystals as scattering optical labels

Bradley R. Smith^*, Marcus Niebert, Taras Plakhotnik, Andrei V. Zvyagin

^*Corresponding author for this work

Research output: Contribution to journal › Article

41 Citations (Scopus)

Abstract

We report on the first demonstration of nanodiamond (ND) as a scattering optical label in a biological environment. NDs were efficiently transfected into cells using cationic liposomes, and imaged using differential interference and Hoffman modulation 'space' contrast microscopy techniques. We have shown that 55 nm NDs are biologically inert and produce a bright signal compared to the cell background. ND as a scattering label presents the possibility for extended biological imaging with relatively little thermal or biochemical perturbations due to the optical transparency and biologically inert nature of diamond.

Original language	English
Pages (from-to)	260-263
Number of pages	4
Journal	Journal of Luminescence
Volume	127
Issue number	1
DOIs	https://doi.org/10.1016/j.jlumin.2007.02.044
Publication status	Published - Nov 2007
Externally published	Yes

Access to Document

10.1016/j.jlumin.2007.02.044

Link to publication in Scopus

Fingerprint Dive into the research topics of 'Transfection and imaging of diamond nanocrystals as scattering optical labels'. Together they form a unique fingerprint.

Cite this

@article{4c001cb2e4d94a29921a60b6ae8c1d8b,

title = "Transfection and imaging of diamond nanocrystals as scattering optical labels",

abstract = "We report on the first demonstration of nanodiamond (ND) as a scattering optical label in a biological environment. NDs were efficiently transfected into cells using cationic liposomes, and imaged using differential interference and Hoffman modulation 'space' contrast microscopy techniques. We have shown that 55 nm NDs are biologically inert and produce a bright signal compared to the cell background. ND as a scattering label presents the possibility for extended biological imaging with relatively little thermal or biochemical perturbations due to the optical transparency and biologically inert nature of diamond.",

author = "Smith, {Bradley R.} and Marcus Niebert and Taras Plakhotnik and Zvyagin, {Andrei V.}",

year = "2007",

month = "11",

doi = "10.1016/j.jlumin.2007.02.044",

language = "English",

volume = "127",

pages = "260--263",

journal = "Journal of Luminescence",

issn = "0022-2313",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Transfection and imaging of diamond nanocrystals as scattering optical labels

AU - Smith, Bradley R.

AU - Niebert, Marcus

AU - Plakhotnik, Taras

AU - Zvyagin, Andrei V.

PY - 2007/11

Y1 - 2007/11

N2 - We report on the first demonstration of nanodiamond (ND) as a scattering optical label in a biological environment. NDs were efficiently transfected into cells using cationic liposomes, and imaged using differential interference and Hoffman modulation 'space' contrast microscopy techniques. We have shown that 55 nm NDs are biologically inert and produce a bright signal compared to the cell background. ND as a scattering label presents the possibility for extended biological imaging with relatively little thermal or biochemical perturbations due to the optical transparency and biologically inert nature of diamond.

AB - We report on the first demonstration of nanodiamond (ND) as a scattering optical label in a biological environment. NDs were efficiently transfected into cells using cationic liposomes, and imaged using differential interference and Hoffman modulation 'space' contrast microscopy techniques. We have shown that 55 nm NDs are biologically inert and produce a bright signal compared to the cell background. ND as a scattering label presents the possibility for extended biological imaging with relatively little thermal or biochemical perturbations due to the optical transparency and biologically inert nature of diamond.

UR - http://www.scopus.com/inward/record.url?scp=34248570028&partnerID=8YFLogxK

U2 - 10.1016/j.jlumin.2007.02.044

DO - 10.1016/j.jlumin.2007.02.044

M3 - Article

VL - 127

SP - 260

EP - 263

JO - Journal of Luminescence

JF - Journal of Luminescence

SN - 0022-2313

IS - 1

ER -