Transfer ribonucleic acids from Escherichia coli treated with 5-fluorouracil

R. J. Lowrie, P. L. Bergquist

Research output: Contribution to journalArticleResearchpeer-review

Abstract

5-Fluorouracil (FU) is readily incorporated into the transfer ribonucleic acids (tRNAs) of Escherichia coli. tRNA synthesized in the presence of the analog may have up to 100% replacement of uracil by FU. The relative amounts of the other three major bases are unchanged but the relative amounts of ribothymidylic and pseudouridylic acids are reduced. Incorporation of FU into tRNA does not affect its ability to accept amino acids or to transfer phenylalanine into polyphenylalanine or lysine into polylysine in an E. coli S-30 system. Transfer of lysine into polypeptides synthesized under the direction of bacteriophage R17 RNA is inhibited by tRNA from FU-treated cells. This inhibition does not appear to be due to errors in codon recognition by lysyl-tRNA containing FU. There was no difference in response to a series of A,G copolymers between lysyl-tRNA containing FU and control lysyl-tRNA in the ribosome binding assay. tRNA containing FU has been separated from contaminating unsubstituted tRNA by chromatography on columns of methylated albumin kieselguhr. tRNA containing FU has been shown to possess an altered secondary structure as judged by its thermal denaturation profile.

LanguageEnglish
Pages1761-1770
Number of pages10
JournalBiochemistry
Volume7
Issue number5
Publication statusPublished - 1968
Externally publishedYes

Fingerprint

Transfer RNA
Fluorouracil
Escherichia coli
Lysine
RNA Phages
Diatomaceous Earth
Denaturation
Bacteriophages
Polylysine
Uracil
Chromatography
Phenylalanine
Ribosomes
Codon
Albumins
Assays
Copolymers
Hot Temperature
Cells
RNA

Cite this

Lowrie, R. J., & Bergquist, P. L. (1968). Transfer ribonucleic acids from Escherichia coli treated with 5-fluorouracil. Biochemistry, 7(5), 1761-1770.
Lowrie, R. J. ; Bergquist, P. L. / Transfer ribonucleic acids from Escherichia coli treated with 5-fluorouracil. In: Biochemistry. 1968 ; Vol. 7, No. 5. pp. 1761-1770.
@article{2abd73876eb54bffad27188506ab0216,
title = "Transfer ribonucleic acids from Escherichia coli treated with 5-fluorouracil",
abstract = "5-Fluorouracil (FU) is readily incorporated into the transfer ribonucleic acids (tRNAs) of Escherichia coli. tRNA synthesized in the presence of the analog may have up to 100{\%} replacement of uracil by FU. The relative amounts of the other three major bases are unchanged but the relative amounts of ribothymidylic and pseudouridylic acids are reduced. Incorporation of FU into tRNA does not affect its ability to accept amino acids or to transfer phenylalanine into polyphenylalanine or lysine into polylysine in an E. coli S-30 system. Transfer of lysine into polypeptides synthesized under the direction of bacteriophage R17 RNA is inhibited by tRNA from FU-treated cells. This inhibition does not appear to be due to errors in codon recognition by lysyl-tRNA containing FU. There was no difference in response to a series of A,G copolymers between lysyl-tRNA containing FU and control lysyl-tRNA in the ribosome binding assay. tRNA containing FU has been separated from contaminating unsubstituted tRNA by chromatography on columns of methylated albumin kieselguhr. tRNA containing FU has been shown to possess an altered secondary structure as judged by its thermal denaturation profile.",
author = "Lowrie, {R. J.} and Bergquist, {P. L.}",
year = "1968",
language = "English",
volume = "7",
pages = "1761--1770",
journal = "Biochemistry",
issn = "0006-2960",
publisher = "American Chemical Society",
number = "5",

}

Lowrie, RJ & Bergquist, PL 1968, 'Transfer ribonucleic acids from Escherichia coli treated with 5-fluorouracil', Biochemistry, vol. 7, no. 5, pp. 1761-1770.

Transfer ribonucleic acids from Escherichia coli treated with 5-fluorouracil. / Lowrie, R. J.; Bergquist, P. L.

In: Biochemistry, Vol. 7, No. 5, 1968, p. 1761-1770.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Transfer ribonucleic acids from Escherichia coli treated with 5-fluorouracil

AU - Lowrie, R. J.

AU - Bergquist, P. L.

PY - 1968

Y1 - 1968

N2 - 5-Fluorouracil (FU) is readily incorporated into the transfer ribonucleic acids (tRNAs) of Escherichia coli. tRNA synthesized in the presence of the analog may have up to 100% replacement of uracil by FU. The relative amounts of the other three major bases are unchanged but the relative amounts of ribothymidylic and pseudouridylic acids are reduced. Incorporation of FU into tRNA does not affect its ability to accept amino acids or to transfer phenylalanine into polyphenylalanine or lysine into polylysine in an E. coli S-30 system. Transfer of lysine into polypeptides synthesized under the direction of bacteriophage R17 RNA is inhibited by tRNA from FU-treated cells. This inhibition does not appear to be due to errors in codon recognition by lysyl-tRNA containing FU. There was no difference in response to a series of A,G copolymers between lysyl-tRNA containing FU and control lysyl-tRNA in the ribosome binding assay. tRNA containing FU has been separated from contaminating unsubstituted tRNA by chromatography on columns of methylated albumin kieselguhr. tRNA containing FU has been shown to possess an altered secondary structure as judged by its thermal denaturation profile.

AB - 5-Fluorouracil (FU) is readily incorporated into the transfer ribonucleic acids (tRNAs) of Escherichia coli. tRNA synthesized in the presence of the analog may have up to 100% replacement of uracil by FU. The relative amounts of the other three major bases are unchanged but the relative amounts of ribothymidylic and pseudouridylic acids are reduced. Incorporation of FU into tRNA does not affect its ability to accept amino acids or to transfer phenylalanine into polyphenylalanine or lysine into polylysine in an E. coli S-30 system. Transfer of lysine into polypeptides synthesized under the direction of bacteriophage R17 RNA is inhibited by tRNA from FU-treated cells. This inhibition does not appear to be due to errors in codon recognition by lysyl-tRNA containing FU. There was no difference in response to a series of A,G copolymers between lysyl-tRNA containing FU and control lysyl-tRNA in the ribosome binding assay. tRNA containing FU has been separated from contaminating unsubstituted tRNA by chromatography on columns of methylated albumin kieselguhr. tRNA containing FU has been shown to possess an altered secondary structure as judged by its thermal denaturation profile.

UR - http://www.scopus.com/inward/record.url?scp=0014287024&partnerID=8YFLogxK

M3 - Article

VL - 7

SP - 1761

EP - 1770

JO - Biochemistry

T2 - Biochemistry

JF - Biochemistry

SN - 0006-2960

IS - 5

ER -