TY - JOUR
T1 - Transients in the photosynthetic carbon reduction cycle produced by iodoacetic acid and ammonium chloride
AU - Hiller, R. G.
PY - 1970/8
Y1 - 1970/8
N2 - Changes in 14C content of metabolites in photosynthesising Chlorella have been investigated following the addition of iodoacetic acid or ammonium chloride. The 14C content of phosphoglyeric acid increases in the former and decreases in the latter case. Both reagents divert 14C from sucrose and polysaccharide into compounds of and associated with the tricarboxylic acid cycle. The principal site of iodoacetic acid inhibition is triose phosphate dehydrogenase whereas ammonium chloride stimulates the conversion of phosphoglyceric acid to phosphoenolpyruvate and pyruciv acid. The latter suggests a direct effect of the ammonium ion on phosphoglyceromutase, enolase, or pyruvic kinase in vivo. Changes in the concentration of alanine, aspartic acid, glutamic acid, serine, and glutamine upon addition of the ammonium ion are not well correlated with their 14C labelling patterns.
AB - Changes in 14C content of metabolites in photosynthesising Chlorella have been investigated following the addition of iodoacetic acid or ammonium chloride. The 14C content of phosphoglyeric acid increases in the former and decreases in the latter case. Both reagents divert 14C from sucrose and polysaccharide into compounds of and associated with the tricarboxylic acid cycle. The principal site of iodoacetic acid inhibition is triose phosphate dehydrogenase whereas ammonium chloride stimulates the conversion of phosphoglyceric acid to phosphoenolpyruvate and pyruciv acid. The latter suggests a direct effect of the ammonium ion on phosphoglyceromutase, enolase, or pyruvic kinase in vivo. Changes in the concentration of alanine, aspartic acid, glutamic acid, serine, and glutamine upon addition of the ammonium ion are not well correlated with their 14C labelling patterns.
UR - http://www.scopus.com/inward/record.url?scp=77956981548&partnerID=8YFLogxK
U2 - 10.1093/jxb/21.3.628
DO - 10.1093/jxb/21.3.628
M3 - Article
AN - SCOPUS:77956981548
SN - 0022-0957
VL - 21
SP - 628
EP - 638
JO - Journal of Experimental Botany
JF - Journal of Experimental Botany
IS - 3
ER -