Transients in the photosynthetic carbon reduction cycle produced by iodoacetic acid and ammonium chloride

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Changes in 14C content of metabolites in photosynthesising Chlorella have been investigated following the addition of iodoacetic acid or ammonium chloride. The 14C content of phosphoglyeric acid increases in the former and decreases in the latter case. Both reagents divert 14C from sucrose and polysaccharide into compounds of and associated with the tricarboxylic acid cycle. The principal site of iodoacetic acid inhibition is triose phosphate dehydrogenase whereas ammonium chloride stimulates the conversion of phosphoglyceric acid to phosphoenolpyruvate and pyruciv acid. The latter suggests a direct effect of the ammonium ion on phosphoglyceromutase, enolase, or pyruvic kinase in vivo. Changes in the concentration of alanine, aspartic acid, glutamic acid, serine, and glutamine upon addition of the ammonium ion are not well correlated with their 14C labelling patterns.

LanguageEnglish
Pages628-638
Number of pages11
JournalJournal of Experimental Botany
Volume21
Issue number3
DOIs
Publication statusPublished - Aug 1970
Externally publishedYes

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iodoacetic acid
Iodoacetic Acid
Carbon Cycle
Ammonium Chloride
ammonium compounds
ammonium chloride
trioses
Ammonium Compounds
phosphoglyceric acids
phosphopyruvate hydratase
Acids
tricarboxylic acid cycle
carbon
acids
Chlorella
aspartic acid
Phosphoglycerate Mutase
Trioses
glutamic acid
glutamine

Cite this

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abstract = "Changes in 14C content of metabolites in photosynthesising Chlorella have been investigated following the addition of iodoacetic acid or ammonium chloride. The 14C content of phosphoglyeric acid increases in the former and decreases in the latter case. Both reagents divert 14C from sucrose and polysaccharide into compounds of and associated with the tricarboxylic acid cycle. The principal site of iodoacetic acid inhibition is triose phosphate dehydrogenase whereas ammonium chloride stimulates the conversion of phosphoglyceric acid to phosphoenolpyruvate and pyruciv acid. The latter suggests a direct effect of the ammonium ion on phosphoglyceromutase, enolase, or pyruvic kinase in vivo. Changes in the concentration of alanine, aspartic acid, glutamic acid, serine, and glutamine upon addition of the ammonium ion are not well correlated with their 14C labelling patterns.",
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Transients in the photosynthetic carbon reduction cycle produced by iodoacetic acid and ammonium chloride. / Hiller, R. G.

In: Journal of Experimental Botany, Vol. 21, No. 3, 08.1970, p. 628-638.

Research output: Contribution to journalArticleResearchpeer-review

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AB - Changes in 14C content of metabolites in photosynthesising Chlorella have been investigated following the addition of iodoacetic acid or ammonium chloride. The 14C content of phosphoglyeric acid increases in the former and decreases in the latter case. Both reagents divert 14C from sucrose and polysaccharide into compounds of and associated with the tricarboxylic acid cycle. The principal site of iodoacetic acid inhibition is triose phosphate dehydrogenase whereas ammonium chloride stimulates the conversion of phosphoglyceric acid to phosphoenolpyruvate and pyruciv acid. The latter suggests a direct effect of the ammonium ion on phosphoglyceromutase, enolase, or pyruvic kinase in vivo. Changes in the concentration of alanine, aspartic acid, glutamic acid, serine, and glutamine upon addition of the ammonium ion are not well correlated with their 14C labelling patterns.

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