Trichoderma reesei RUT-C30 - Thirty years of strain improvement

Robyn Peterson*, Helena Nevalainen

*Corresponding author for this work

    Research output: Contribution to journalReview articlepeer-review

    354 Citations (Scopus)


    The hypersecreting mutant Trichoderma reesei RUT-C30 (ATCC 56765) is one of the most widely used strains of filamentous fungi for the production of cellulolytic enzymes and recombinant proteins, and for academic research. The strain was obtained after three rounds of random mutagenesis of the wild-type QM6a in a screening program focused on high cellulase production and catabolite derepression. Whereas RUT-C30 achieves outstanding levels of protein secretion and high cellulolytic activity in comparison to the wild-type QM6a, recombinant protein production has been less successful. Here, we bring together and discuss the results from biochemical-, microscopic-, genomic-, transcriptomic-, glycomic- and proteomic-based research on the RUTC30 strain published over the last 30 years.

    Original languageEnglish
    Pages (from-to)58-68
    Number of pages11
    Publication statusPublished - Jan 2012


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