TY - JOUR
T1 - Trimming the genomic fat
T2 - minimising and re-functionalising genomes using synthetic biology
AU - Xu, Xin
AU - Meier, Felix
AU - Blount, Benjamin A.
AU - Pretorius, Isak S.
AU - Ellis, Tom
AU - Paulsen, Ian T.
AU - Williams, Thomas C.
N1 - Copyright the Author(s) 2023. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.
PY - 2023/4/8
Y1 - 2023/4/8
N2 - Naturally evolved organisms typically have large genomes that enable their survival and growth under various conditions. However, the complexity of genomes often precludes our complete understanding of them, and limits the success of biotechnological designs. In contrast, minimal genomes have reduced complexity and therefore improved engineerability, increased biosynthetic capacity through the removal of unnecessary genetic elements, and less recalcitrance to complete characterisation. Here, we review the past and current genome minimisation and re-functionalisation efforts, with an emphasis on the latest advances facilitated by synthetic genomics, and provide a critical appraisal of their potential for industrial applications.
AB - Naturally evolved organisms typically have large genomes that enable their survival and growth under various conditions. However, the complexity of genomes often precludes our complete understanding of them, and limits the success of biotechnological designs. In contrast, minimal genomes have reduced complexity and therefore improved engineerability, increased biosynthetic capacity through the removal of unnecessary genetic elements, and less recalcitrance to complete characterisation. Here, we review the past and current genome minimisation and re-functionalisation efforts, with an emphasis on the latest advances facilitated by synthetic genomics, and provide a critical appraisal of their potential for industrial applications.
UR - http://www.scopus.com/inward/record.url?scp=85151979635&partnerID=8YFLogxK
U2 - 10.1038/s41467-023-37748-7
DO - 10.1038/s41467-023-37748-7
M3 - Review article
C2 - 37031253
AN - SCOPUS:85151979635
SN - 2041-1723
VL - 14
SP - 1
EP - 11
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 1984
ER -