Tropomyosin isoforms have specific effects on the transcriptome of undifferentiated and differentiated B35 neuroblastoma cells

Holly Stefen, Alexandra Kalyna Suchowerska, Bei Jun Chen, Merryn Brettle, Jennifer Kuschelewski, Peter William Gunning, Michael Janitz, Thomas Fath*

*Corresponding author for this work

Research output: Contribution to journalArticle

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Abstract

Tropomyosins, a family of actin-associated proteins, bestow actin filaments with distinct biochemical and physical properties which are important for determining cell shape and regulating many cellular processes in eukaryotic cells. Here, we used RNA-seq to investigate the effect of four tropomyosin isoforms on gene expression in undifferentiated and differentiated rat B35 neuroblastoma cells. In undifferentiated cells, overexpression of tropomyosin isoforms Tpm1.12, Tpm2.1, Tpm3.1, and Tpm4.2 differentially regulates a vast number of genes, clustering into several gene ontology terms. In differentiated cells, tropomyosin overexpression exerts a much weaker influence on overall gene expression. Our findings are particularly compelling because they demonstrate that tropomyosin-dependent changes are attenuated once the cells are induced to follow a defined path of differentiation. 

Database: Sequence data for public availability are deposited in the European Nucleotide Archive under the accession number PRJEB24136.

Original languageEnglish
Pages (from-to)570-583
Number of pages14
JournalFEBS Open Bio
Volume8
Issue number4
DOIs
Publication statusPublished - 1 Apr 2018
Externally publishedYes

Bibliographical note

Copyright the Author(s) 2018. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

  • actin cytoskeleton
  • RNA-seq
  • tropomyosin isoforms

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