TRPA1: A Potential Prognostic Indicator for Oral Squamous Cell Carcinoma

Rajdeep Chakraborty; Amara Jabeen; Honghua Hu; Charbel Darido; Karen Vickery; Shoba Ranganathan

doi:10.1101/2021.11.29.470496

TRPA1: A Potential Prognostic Indicator for Oral Squamous Cell Carcinoma

Rajdeep Chakraborty, Amara Jabeen, Honghua Hu, Charbel Darido, Karen Vickery, Shoba Ranganathan

Research output: Contribution to journal › Other journal contribution

Abstract

Introduction: Transient receptors are related to oral cancer pain. Previously capsaicin (TRPV1 agonist) was shown to induce cell death in oral cancer cells. We hypothesised that these receptors are present in oral cancer. Method: We examined the presence of cannabinoid receptors (CB1 and CB2) and targets (TRPV1, TRPA1, CaV 3.1, CaV 3.2, CaV 3.3) via quantitative polymerase chain reaction (qPCR) in oral cancer cells SCC4, SCC9, SCC25, Cal27, and normal oral cell line OKF6. Result: Cannabinoid receptors are absent in all the cell lines, while TRPA1 is only present in normal cells, but absent in all the oral cancer cell lines. Voltage-gated calcium channels are present in all the cell lines. Conclusion and Future Aspects: TRPA1 could be the possible future prognostic indicator of oral squamous cell carcinoma. Future functionality assays could use precancerous cell lines to follow the loss of TRPA1.

Original language	English
Journal	bioRxiv
DOIs	https://doi.org/10.1101/2021.11.29.470496
Publication status	Submitted - 30 Nov 2021

Keywords

TRPA1
Oral Cancer cells
Oral Squamous cell carcinoma
Cannabinoids
TRPV1
Voltage Gated Calcium channels
Cannabinoid receptors

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@article{154d8eaf6c8c4bacb7067a0b7b55943e,

title = "TRPA1: A Potential Prognostic Indicator for Oral Squamous Cell Carcinoma",

abstract = "Introduction: Transient receptors are related to oral cancer pain. Previously capsaicin (TRPV1 agonist) was shown to induce cell death in oral cancer cells. We hypothesised that these receptors are present in oral cancer. Method: We examined the presence of cannabinoid receptors (CB1 and CB2) and targets (TRPV1, TRPA1, CaV 3.1, CaV 3.2, CaV 3.3) via quantitative polymerase chain reaction (qPCR) in oral cancer cells SCC4, SCC9, SCC25, Cal27, and normal oral cell line OKF6. Result: Cannabinoid receptors are absent in all the cell lines, while TRPA1 is only present in normal cells, but absent in all the oral cancer cell lines. Voltage-gated calcium channels are present in all the cell lines. Conclusion and Future Aspects: TRPA1 could be the possible future prognostic indicator of oral squamous cell carcinoma. Future functionality assays could use precancerous cell lines to follow the loss of TRPA1.",

keywords = "TRPA1, Oral Cancer cells, Oral Squamous cell carcinoma, Cannabinoids, TRPV1, Voltage Gated Calcium channels, Cannabinoid receptors",

author = "Rajdeep Chakraborty and Amara Jabeen and Honghua Hu and Charbel Darido and Karen Vickery and Shoba Ranganathan",

year = "2021",

month = nov,

day = "30",

doi = "10.1101/2021.11.29.470496",

language = "English",

journal = "bioRxiv",

}

TY - JOUR

T1 - TRPA1: A Potential Prognostic Indicator for Oral Squamous Cell Carcinoma

AU - Chakraborty, Rajdeep

AU - Jabeen, Amara

AU - Hu, Honghua

AU - Darido, Charbel

AU - Vickery, Karen

AU - Ranganathan, Shoba

PY - 2021/11/30

Y1 - 2021/11/30

N2 - Introduction: Transient receptors are related to oral cancer pain. Previously capsaicin (TRPV1 agonist) was shown to induce cell death in oral cancer cells. We hypothesised that these receptors are present in oral cancer. Method: We examined the presence of cannabinoid receptors (CB1 and CB2) and targets (TRPV1, TRPA1, CaV 3.1, CaV 3.2, CaV 3.3) via quantitative polymerase chain reaction (qPCR) in oral cancer cells SCC4, SCC9, SCC25, Cal27, and normal oral cell line OKF6. Result: Cannabinoid receptors are absent in all the cell lines, while TRPA1 is only present in normal cells, but absent in all the oral cancer cell lines. Voltage-gated calcium channels are present in all the cell lines. Conclusion and Future Aspects: TRPA1 could be the possible future prognostic indicator of oral squamous cell carcinoma. Future functionality assays could use precancerous cell lines to follow the loss of TRPA1.

AB - Introduction: Transient receptors are related to oral cancer pain. Previously capsaicin (TRPV1 agonist) was shown to induce cell death in oral cancer cells. We hypothesised that these receptors are present in oral cancer. Method: We examined the presence of cannabinoid receptors (CB1 and CB2) and targets (TRPV1, TRPA1, CaV 3.1, CaV 3.2, CaV 3.3) via quantitative polymerase chain reaction (qPCR) in oral cancer cells SCC4, SCC9, SCC25, Cal27, and normal oral cell line OKF6. Result: Cannabinoid receptors are absent in all the cell lines, while TRPA1 is only present in normal cells, but absent in all the oral cancer cell lines. Voltage-gated calcium channels are present in all the cell lines. Conclusion and Future Aspects: TRPA1 could be the possible future prognostic indicator of oral squamous cell carcinoma. Future functionality assays could use precancerous cell lines to follow the loss of TRPA1.

KW - TRPA1

KW - Oral Cancer cells

KW - Oral Squamous cell carcinoma

KW - Cannabinoids

KW - TRPV1

KW - Voltage Gated Calcium channels

KW - Cannabinoid receptors

U2 - 10.1101/2021.11.29.470496

DO - 10.1101/2021.11.29.470496

M3 - Other journal contribution

JO - bioRxiv

JF - bioRxiv

ER -

TRPA1: A Potential Prognostic Indicator for Oral Squamous Cell Carcinoma

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Keywords

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