TrpA1 Regulates Thermal Nociception in Drosophila

G. Gregory Neely*, Alex C. Keene, Peter Duchek, Elaine C. Chang, Qiao-Ping Wang, Yagiz Alp Aksoy, Mark Rosenzweig, Michael Costigan, Clifford J. Woolf, Paul A. Garrity, Josef M. Penninger

*Corresponding author for this work

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

Pain is a significant medical concern and represents a major unmet clinical need. The ability to perceive and react to tissue-damaging stimuli is essential in order to maintain bodily integrity in the face of environmental danger. To prevent damage the systems that detect noxious stimuli are therefore under strict evolutionary pressure. We developed a high-throughput behavioral method to identify genes contributing to thermal nociception in the fruit fly and have reported a large-scale screen that identified the Ca(2+) channel straightjacket (stj) as a conserved regulator of thermal nociception. Here we present the minimal anatomical and neuronal requirements for Drosophila to avoid noxious heat in our novel behavioral paradigm. Bioinformatics analysis of our whole genome data set revealed 23 genes implicated in Ca(2+) signaling that are required for noxious heat avoidance. One of these genes, the conserved thermoreceptor TrpA1, was confirmed as a bona fide "pain'' gene in both adult and larval fly nociception paradigms. The nociceptive function of TrpA1 required expression within the Drosophila nervous system, specifically within nociceptive multi-dendritic (MD) sensory neurons. Therefore, our analysis identifies the channel TRPA1 as a conserved regulator of nociception.

Original languageEnglish
Article number24343
Pages (from-to)1-9
Number of pages9
JournalPLoS ONE
Volume6
Issue number8
DOIs
Publication statusPublished - 31 Aug 2011
Externally publishedYes

Keywords

  • TEMPERATURE PREFERENCE
  • CHEMICAL NOCICEPTION
  • ADULT DROSOPHILA
  • MUSHROOM BODIES
  • GENE
  • PAIN
  • MEMORY
  • COLD
  • CONTRIBUTES
  • BEHAVIOR

Cite this

Neely, G. G., Keene, A. C., Duchek, P., Chang, E. C., Wang, Q-P., Aksoy, Y. A., ... Penninger, J. M. (2011). TrpA1 Regulates Thermal Nociception in Drosophila. PLoS ONE, 6(8), 1-9. [24343]. https://doi.org/10.1371/journal.pone.0024343