Abstract
We report an aggregation-induced emission fluorogen (AIEgen)-based turn-on fluorescent aptasensor able to detect the ultrasmall concentration of intracellular IFN-γ. The aptasensor consists of an IFN-γ aptamer labeled with a fluorogen with a typical aggregation-induced emission (AIE) characteristic, which shows strong red emission only in the presence of IFN-γ. The aptasensor is able to effectively monitor intracellular IFN-γ secretion with the lowest detection limit of 2 pg mL⁻¹, and it is capable of localizing IFN-γ in live cells during secretion, with excellent cellular permeability and biocompatibility as well as low cytotoxicity. This probe is able to localize the intracellular IFN-γ at a low concentration <10 pg mL⁻¹, and it is successfully used for real-time bioimaging. This simple and highly sensitive sensor may enable the exploration of cytokine pathways and their dynamic secretion process in the cellular environment. It provides a universal sensing platform for monitoring a spectrum of molecules secreted by cells.
| Original language | English |
|---|---|
| Pages (from-to) | 320–326 |
| Number of pages | 7 |
| Journal | ACS Sensors |
| Volume | 3 |
| Issue number | 2 |
| Early online date | 8 Jan 2018 |
| DOIs | |
| Publication status | Published - Feb 2018 |
Keywords
- IFN-γ detection
- AIE fluorogens
- aptasensor
- bioimaging
- turn-on fluorescence
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Ma, K., Zhang, F., Sayyadi, N., Chen, W., Anwer, A. G., Care, A., ... Liu, G. (2018). "Turn-on" fluorescent aptasensor based on AIEgen labeling for the localization of IFN-γ in live cells. ACS Sensors, 3(2), 320–326. https://doi.org/10.1021/acssensors.7b00720