Abstract
| Language | English |
|---|---|
| Pages | 320–326 |
| Number of pages | 7 |
| Journal | ACS Sensors |
| Volume | 3 |
| Issue number | 2 |
| Early online date | 8 Jan 2018 |
| DOIs | |
| Publication status | Published - Feb 2018 |
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Keywords
- IFN-γ detection
- AIE fluorogens
- aptasensor
- bioimaging
- turn-on fluorescence
Cite this
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"Turn-on" fluorescent aptasensor based on AIEgen labeling for the localization of IFN-γ in live cells. / Ma, Ke; Zhang, Fengli; Sayyadi, Nima; Chen, Wenjie; Anwer, Ayad G.; Care, Andrew; Xu, Bin; Tian, Wenjing; Goldys, Ewa M.; Liu, Guozhen.
In: ACS Sensors, Vol. 3, No. 2, 02.2018, p. 320–326.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - "Turn-on" fluorescent aptasensor based on AIEgen labeling for the localization of IFN-γ in live cells
AU - Ma, Ke
AU - Zhang, Fengli
AU - Sayyadi, Nima
AU - Chen, Wenjie
AU - Anwer, Ayad G.
AU - Care, Andrew
AU - Xu, Bin
AU - Tian, Wenjing
AU - Goldys, Ewa M.
AU - Liu, Guozhen
PY - 2018/2
Y1 - 2018/2
N2 - We report an aggregation-induced emission fluorogen (AIEgen)-based turn-on fluorescent aptasensor able to detect the ultrasmall concentration of intracellular IFN-γ. The aptasensor consists of an IFN-γ aptamer labeled with a fluorogen with a typical aggregation-induced emission (AIE) characteristic, which shows strong red emission only in the presence of IFN-γ. The aptasensor is able to effectively monitor intracellular IFN-γ secretion with the lowest detection limit of 2 pg mL⁻¹, and it is capable of localizing IFN-γ in live cells during secretion, with excellent cellular permeability and biocompatibility as well as low cytotoxicity. This probe is able to localize the intracellular IFN-γ at a low concentration <10 pg mL⁻¹, and it is successfully used for real-time bioimaging. This simple and highly sensitive sensor may enable the exploration of cytokine pathways and their dynamic secretion process in the cellular environment. It provides a universal sensing platform for monitoring a spectrum of molecules secreted by cells.
AB - We report an aggregation-induced emission fluorogen (AIEgen)-based turn-on fluorescent aptasensor able to detect the ultrasmall concentration of intracellular IFN-γ. The aptasensor consists of an IFN-γ aptamer labeled with a fluorogen with a typical aggregation-induced emission (AIE) characteristic, which shows strong red emission only in the presence of IFN-γ. The aptasensor is able to effectively monitor intracellular IFN-γ secretion with the lowest detection limit of 2 pg mL⁻¹, and it is capable of localizing IFN-γ in live cells during secretion, with excellent cellular permeability and biocompatibility as well as low cytotoxicity. This probe is able to localize the intracellular IFN-γ at a low concentration <10 pg mL⁻¹, and it is successfully used for real-time bioimaging. This simple and highly sensitive sensor may enable the exploration of cytokine pathways and their dynamic secretion process in the cellular environment. It provides a universal sensing platform for monitoring a spectrum of molecules secreted by cells.
KW - IFN-γ detection
KW - AIE fluorogens
KW - aptasensor
KW - bioimaging
KW - turn-on fluorescence
UR - http://purl.org/au-research/grants/arc/CE140100003
UR - http://www.scopus.com/inward/record.url?scp=85042545348&partnerID=8YFLogxK
U2 - 10.1021/acssensors.7b00720
DO - 10.1021/acssensors.7b00720
M3 - Article
VL - 3
SP - 320
EP - 326
JO - ACS Sensors
T2 - ACS Sensors
JF - ACS Sensors
SN - 2379-3694
IS - 2
ER -