"Turn-on" fluorescent aptasensor based on AIEgen labeling for the localization of IFN-γ in live cells

Ke Ma; Fengli Zhang; Nima Sayyadi; Wenjie Chen; Ayad G. Anwer; Andrew Care; Bin Xu; Wenjing Tian; Ewa M. Goldys; Guozhen Liu

doi:10.1021/acssensors.7b00720

"Turn-on" fluorescent aptasensor based on AIEgen labeling for the localization of IFN-γ in live cells

Ke Ma, Fengli Zhang, Nima Sayyadi, Wenjie Chen, Ayad G. Anwer, Andrew Care, Bin Xu, Wenjing Tian, Ewa M. Goldys, Guozhen Liu

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

We report an aggregation-induced emission fluorogen (AIEgen)-based turn-on fluorescent aptasensor able to detect the ultrasmall concentration of intracellular IFN-γ. The aptasensor consists of an IFN-γ aptamer labeled with a fluorogen with a typical aggregation-induced emission (AIE) characteristic, which shows strong red emission only in the presence of IFN-γ. The aptasensor is able to effectively monitor intracellular IFN-γ secretion with the lowest detection limit of 2 pg mL⁻¹, and it is capable of localizing IFN-γ in live cells during secretion, with excellent cellular permeability and biocompatibility as well as low cytotoxicity. This probe is able to localize the intracellular IFN-γ at a low concentration <10 pg mL⁻¹, and it is successfully used for real-time bioimaging. This simple and highly sensitive sensor may enable the exploration of cytokine pathways and their dynamic secretion process in the cellular environment. It provides a universal sensing platform for monitoring a spectrum of molecules secreted by cells.

Original language	English
Pages (from-to)	320–326
Number of pages	7
Journal	ACS Sensors
Volume	3
Issue number	2
Early online date	8 Jan 2018
DOIs	https://doi.org/10.1021/acssensors.7b00720
Publication status	Published - Feb 2018

Keywords

IFN-γ detection
AIE fluorogens
aptasensor
bioimaging
turn-on fluorescence

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10.1021/acssensors.7b00720

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title = "{"}Turn-on{"} fluorescent aptasensor based on AIEgen labeling for the localization of IFN-γ in live cells",

abstract = "We report an aggregation-induced emission fluorogen (AIEgen)-based turn-on fluorescent aptasensor able to detect the ultrasmall concentration of intracellular IFN-γ. The aptasensor consists of an IFN-γ aptamer labeled with a fluorogen with a typical aggregation-induced emission (AIE) characteristic, which shows strong red emission only in the presence of IFN-γ. The aptasensor is able to effectively monitor intracellular IFN-γ secretion with the lowest detection limit of 2 pg mL⁻¹, and it is capable of localizing IFN-γ in live cells during secretion, with excellent cellular permeability and biocompatibility as well as low cytotoxicity. This probe is able to localize the intracellular IFN-γ at a low concentration <10 pg mL⁻¹, and it is successfully used for real-time bioimaging. This simple and highly sensitive sensor may enable the exploration of cytokine pathways and their dynamic secretion process in the cellular environment. It provides a universal sensing platform for monitoring a spectrum of molecules secreted by cells.",

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author = "Ke Ma and Fengli Zhang and Nima Sayyadi and Wenjie Chen and Anwer, {Ayad G.} and Andrew Care and Bin Xu and Wenjing Tian and Goldys, {Ewa M.} and Guozhen Liu",

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AU - Ma, Ke

AU - Zhang, Fengli

AU - Sayyadi, Nima

AU - Chen, Wenjie

AU - Anwer, Ayad G.

AU - Care, Andrew

AU - Xu, Bin

AU - Tian, Wenjing

AU - Goldys, Ewa M.

AU - Liu, Guozhen

PY - 2018/2

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N2 - We report an aggregation-induced emission fluorogen (AIEgen)-based turn-on fluorescent aptasensor able to detect the ultrasmall concentration of intracellular IFN-γ. The aptasensor consists of an IFN-γ aptamer labeled with a fluorogen with a typical aggregation-induced emission (AIE) characteristic, which shows strong red emission only in the presence of IFN-γ. The aptasensor is able to effectively monitor intracellular IFN-γ secretion with the lowest detection limit of 2 pg mL⁻¹, and it is capable of localizing IFN-γ in live cells during secretion, with excellent cellular permeability and biocompatibility as well as low cytotoxicity. This probe is able to localize the intracellular IFN-γ at a low concentration <10 pg mL⁻¹, and it is successfully used for real-time bioimaging. This simple and highly sensitive sensor may enable the exploration of cytokine pathways and their dynamic secretion process in the cellular environment. It provides a universal sensing platform for monitoring a spectrum of molecules secreted by cells.

AB - We report an aggregation-induced emission fluorogen (AIEgen)-based turn-on fluorescent aptasensor able to detect the ultrasmall concentration of intracellular IFN-γ. The aptasensor consists of an IFN-γ aptamer labeled with a fluorogen with a typical aggregation-induced emission (AIE) characteristic, which shows strong red emission only in the presence of IFN-γ. The aptasensor is able to effectively monitor intracellular IFN-γ secretion with the lowest detection limit of 2 pg mL⁻¹, and it is capable of localizing IFN-γ in live cells during secretion, with excellent cellular permeability and biocompatibility as well as low cytotoxicity. This probe is able to localize the intracellular IFN-γ at a low concentration <10 pg mL⁻¹, and it is successfully used for real-time bioimaging. This simple and highly sensitive sensor may enable the exploration of cytokine pathways and their dynamic secretion process in the cellular environment. It provides a universal sensing platform for monitoring a spectrum of molecules secreted by cells.

KW - IFN-γ detection

KW - AIE fluorogens

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KW - bioimaging

KW - turn-on fluorescence

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