Twenty-five new polymorphic microsatellites for the eastern mosquitofish, Gambusia holbrooki (Actinopterygii: Poeciliidae), an invasive species in Australia

Kate D L Umbers*, Michael D. Jennions, Michael G. Gardner, J. Scott Keogh

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

We isolated 25 new polymorphic microsatellite markers from the eastern mosquitofish, Gambusia holbrooki. Initially, 454 shotgun sequencing was used to identify 1187 loci for which primers could be designed. Of these 1187, we trialled 48 in the target species, 40 of which amplified a product of expected size. Subsequently, those 40 loci were screened for variation in 48 individuals from a single population in Canberra, Australia. Twenty loci were in Hardy-Weinberg equilibrium and polymorphic, with observed heterozygosity ranging from 0.04 to 0.72 (mean: 0.45±0.18) and the number of alleles per locus ranged from 2 to 5 (mean: 3.20±1.05). These loci will be useful in understanding genetic variation, paternity analysis and in managing this species across both its native and invasive range.

Original languageEnglish
Pages (from-to)235-237
Number of pages3
JournalAustralian Journal of Zoology
Volume60
Issue number4
DOIs
Publication statusPublished - 2012

Keywords

  • 454 GS-FLX
  • shotgun sequencing.

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