Two-dimensional gel electrophoresis

Mark P. Molloy; Michael T. McDowell

doi:10.1016/S0149-6395(05)80009-1

Two-dimensional gel electrophoresis

Mark P. Molloy^*, Michael T. McDowell

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter

1 Citation (Scopus)

Abstract

2-DE continues to set the benchmark as the premier technique for protein separation in proteomic applications. The approach is very well suited for routine separation of moderately abundant proteins within a window of pH 3-10 and M_r = 10-200 kDa, allowing for cataloging and quantitative measures of protein expression. The method is mature, reproducible, and affordable.

Original language	English
Title of host publication	Two-dimensional gel electrophoresis
Editors	D Garfin, S Ahuja
Place of Publication	San Diego, CA
Publisher	ELSEVIER ACADEMIC PRESS INC
Pages	123-145
Number of pages	23
ISBN (Print)	9780120887521
DOIs	https://doi.org/10.1016/S0149-6395(05)80009-1
Publication status	Published - 2005
Externally published	Yes

Publication series

Name	Separation Science and Technology
Publisher	ELSEVIER ACADEMIC PRESS INC
Volume	7
ISSN (Print)	1877-1718

Access to Document

10.1016/S0149-6395(05)80009-1

Link to publication in Scopus

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Cite this

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