Two pathogens of greenshell™ mussel larvae, perna canaliculus

Vibrio splendidus and a V. coralliilyticus/neptunius-like isolate

A. Kesarcodi-Watson*, H. Kaspar, M. J. Lategan, L. Gibson

*Corresponding author for this work

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Bacterial pathogens of Greenshell™ mussel (GSM) larvae can cause batch losses during hatchery production. Twenty-two isolates were screened using a larval bioassay. Two strains were identified as potential pathogens. Phenotypic identification of these strains revealed two non-reactive Gram-negative, oxidase positive rods. Sequencing of the 16S rRNA gene identified Vibrio splendidus and a V. coralliilyticus/neptunius-like isolate as pathogens of GSM larvae, with an ability to cause 83% and 75% larval mortality in vitro, respectively, at a concentration of 102 CFU mL-1. Histopathology indicated that the route of infection was via the digestive system. Using healthy larvae as target hosts, Koch's postulates were confirmed for the two isolates. This is the first report on pathogens of GSM larvae.

Original languageEnglish
Pages (from-to)499-507
Number of pages9
JournalJournal of Fish Diseases
Volume32
Issue number6
DOIs
Publication statusPublished - Jun 2009
Externally publishedYes

Fingerprint Dive into the research topics of 'Two pathogens of greenshell™ mussel larvae, perna canaliculus: Vibrio splendidus and a V. coralliilyticus/neptunius-like isolate'. Together they form a unique fingerprint.

Cite this