Two-Step purification of pathogenesis-related proteins from grape juice and crystallization of thaumatin-like proteins

Steven C. Van Sluyter; Matteo Marangon; Samuel D. Stranks; Karlie A. Neilson; Yoji Hayasaka; Paul A. Haynes; R. Ian Menz; Elizabeth J. Waters

doi:10.1021/jf902365r

Two-Step purification of pathogenesis-related proteins from grape juice and crystallization of thaumatin-like proteins

Steven C. Van Sluyter, Matteo Marangon, Samuel D. Stranks, Karlie A. Neilson, Yoji Hayasaka, Paul A. Haynes, R. Ian Menz, Elizabeth J. Waters

Research output: Contribution to journal › Article › peer-review

47 Citations (Scopus)

Abstract

Grape thaumatin-like (TL) proteins and chitinases play roles in plant-pathogen interactions and can cause protein haze in white wine unless removed prior to bottling. A two-step method is described that highly purified hundreds of milligrams of TL proteins and chitinases from two juices by strong cation exchange (SCX) and hydrophobic interaction chromatography (HIC). The method was fast and separated isoforms of TL proteins and chitinases from within the same juice, in most cases to >97% purity. The isolated proteins were identified by peptide nanoLC-MS/MS and crystallized using a high-throughput screening method. Crystals from three protein fractions produced highresolution X-ray crystallography data.

Original language	English
Pages (from-to)	11376-11380
Number of pages	5
Journal	Journal of Agricultural and Food Chemistry
Volume	57
Issue number	23
DOIs	https://doi.org/10.1021/jf902365r
Publication status	Published - 9 Dec 2009

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abstract = "Grape thaumatin-like (TL) proteins and chitinases play roles in plant-pathogen interactions and can cause protein haze in white wine unless removed prior to bottling. A two-step method is described that highly purified hundreds of milligrams of TL proteins and chitinases from two juices by strong cation exchange (SCX) and hydrophobic interaction chromatography (HIC). The method was fast and separated isoforms of TL proteins and chitinases from within the same juice, in most cases to >97% purity. The isolated proteins were identified by peptide nanoLC-MS/MS and crystallized using a high-throughput screening method. Crystals from three protein fractions produced highresolution X-ray crystallography data.",

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AU - Van Sluyter, Steven C.

AU - Marangon, Matteo

AU - Stranks, Samuel D.

AU - Neilson, Karlie A.

AU - Hayasaka, Yoji

AU - Haynes, Paul A.

AU - Ian Menz, R.

AU - Waters, Elizabeth J.

PY - 2009/12/9

Y1 - 2009/12/9

N2 - Grape thaumatin-like (TL) proteins and chitinases play roles in plant-pathogen interactions and can cause protein haze in white wine unless removed prior to bottling. A two-step method is described that highly purified hundreds of milligrams of TL proteins and chitinases from two juices by strong cation exchange (SCX) and hydrophobic interaction chromatography (HIC). The method was fast and separated isoforms of TL proteins and chitinases from within the same juice, in most cases to >97% purity. The isolated proteins were identified by peptide nanoLC-MS/MS and crystallized using a high-throughput screening method. Crystals from three protein fractions produced highresolution X-ray crystallography data.

AB - Grape thaumatin-like (TL) proteins and chitinases play roles in plant-pathogen interactions and can cause protein haze in white wine unless removed prior to bottling. A two-step method is described that highly purified hundreds of milligrams of TL proteins and chitinases from two juices by strong cation exchange (SCX) and hydrophobic interaction chromatography (HIC). The method was fast and separated isoforms of TL proteins and chitinases from within the same juice, in most cases to >97% purity. The isolated proteins were identified by peptide nanoLC-MS/MS and crystallized using a high-throughput screening method. Crystals from three protein fractions produced highresolution X-ray crystallography data.

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Two-Step purification of pathogenesis-related proteins from grape juice and crystallization of thaumatin-like proteins

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