Two-stepping to increase peptide spectra matches in large databases

Mark P. Molloy

doi:10.1002/pmic.201300094

Two-stepping to increase peptide spectra matches in large databases

Mark P. Molloy^*

^*Corresponding author for this work

Research output: Contribution to journal › Comment/opinion › peer-review

Abstract

Metaproteomics and proteogenomic studies face unique challenges in peptide spectra identification due to the very large number of sequences in search databases. Conventional, stringent filtering needed to avoid false positives limits results in these studies due to increasing false negatives, that is, poor sensitivity. Jagtap et al. (Proteomics 2013, 13, 1352-1357) find a solution to this issue by employing a facile two-step search strategy.

Original language	English
Pages (from-to)	1229-1230
Number of pages	2
Journal	Proteomics
Volume	13
Issue number	8
DOIs	https://doi.org/10.1002/pmic.201300094
Publication status	Published - Apr 2013

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title = "Two-stepping to increase peptide spectra matches in large databases",

abstract = "Metaproteomics and proteogenomic studies face unique challenges in peptide spectra identification due to the very large number of sequences in search databases. Conventional, stringent filtering needed to avoid false positives limits results in these studies due to increasing false negatives, that is, poor sensitivity. Jagtap et al. (Proteomics 2013, 13, 1352-1357) find a solution to this issue by employing a facile two-step search strategy.",

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Two-stepping to increase peptide spectra matches in large databases

Abstract

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