Ultra-sensitive time-resolved nanoliter volume fluorometry based on UV LEDs and a channel photomultiplier tube

Dayong Jin, Russell Connally, Jim Piper

Research output: Contribution to journalArticle

4 Citations (Scopus)
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Abstract

A capillary fluorometer was constructed using a 2 mW, 365 nm ultraviolet (UV) light emitting diode (LED) as the excitation source and a new-generation high-gain (3×108) channel photomultiplier tube. The use of a LED permitted rapid pulsing of the excitation source so that the instrument could be employed for time-resolved fluorescence (TRF) applications. A detection limit of ∼2×108 molecules of BHHT (4,4′-bis (1″,1″,1″,2″,2″,3″,3″-heptafluoro- 4″,6″-hexanedion-6″yl)-o-tephenyl)-Eu (III) were resolved within a 1.25 nanoliter volume at a S/N ratio of 3:1. Ultimate sensitivity of the system was compromised due to visible luminescence emitted by the UV LED, centred around 550 nm extending to > 700 nm and 2nd-order exponentially decaying with lifetimes of 40 μs and 490 μs.

Original languageEnglish
Article number36
Pages (from-to)237-245
Number of pages9
JournalProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume5699
DOIs
Publication statusPublished - 2005

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