Ultrastructural Mapping of the Zebrafish gastrointestinal system as a basis for experimental drug studies

Delfine Cheng*, Gerald J. Shami, Marco Morsch, Roger S. Chung, Filip Braet

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)
38 Downloads (Pure)

Abstract

Research in the field of gastroenterology is increasingly focused on the use of alternative nonrodent model organisms to provide new experimental tools to study chronic diseases. The zebrafish is a particularly valuable experimental platform to explore organ and cell structure-function relationships under relevant biological and pathobiological settings. This is due to its optical transparency and its close-to-human genetic makeup. To-date, the structure-function properties of the GIS of the zebrafish are relatively unexplored and limited to histology and fluorescent microscopy. Occasionally those studies include EM of a given subcellular process but lack the required full histological picture. In this work, we employed a novel combined biomolecular imaging approach in order to cross-correlate 3D ultrastructure over different length scales (optical-, X-ray micro-CT, and high-resolution EM). Our correlated imaging studies and subsequent data modelling provide to our knowledge the first detailed 3D picture of the zebrafish larvae GIS. Our results provide unequivocally a limit of confidence for studying various digestive disorders and drug delivery pathways in the zebrafish.

Original languageEnglish
Article number8758460
Pages (from-to)1-13
Number of pages13
JournalBioMed Research International
Volume2016
DOIs
Publication statusPublished - 2016

Bibliographical note

Copyright the Author(s) 2016. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

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