Unlocking the potential of molecularly imprinted polymer-based early detection of creatinine rise - a prognostic leap towards enhanced kidney healthcare

This study introduces a novel approach for detecting creatinine levels in heat-inactivated serum samples using Electrochemical Impedance Spectroscopy (EIS) and Molecularly Imprinted Polymer (MIP) technology. The structural and functional properties of MIP and Non-Molecularly Imprinted Polymer (NIP) materials were thoroughly investigated using Ultra-High Performance Liquid Chromatography (UHPLC) technique. Specifically, the MIP polymers demonstrated exceptional selectivity towards creatinine and structurally related compounds, as validated by UHPLC. Ethical considerations were addressed by utilizing heat-inactivated human serum samples in the testing phase. The developed biosensor exhibits robust specificity, high selectivity, and efficient rebinding capability for the target creatinine molecule. Detection limits were determined to be as low as 0.1 parts per million (ppm). At the same time, the biosensor demonstrated the ability to detect concentrations as high as 50 ppm, which is three times the typical level found in human serum. This research contributes to advancing biosensor technology for precise and sensitive detection of creatinine, promising significant applications in clinical diagnostics and biomedical research. The findings underscore the potential of MIP-based biosensors in enhancing analytical methodologies for biomarker detection and monitoring in medical settings.