Use of SSCP to improve the efficiency of microsatellite identification from microsatellite-enriched libraries

Maxine P. Piggott, Sam C. Banks, Luciano B. Beheregaray*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

An inefficient aspect of marker identification from microsatellite-enriched libraries is the proportion of clones with identical sequences. This can substantially increase the number of clones that need to be sequenced in order to identify a sufficient number of microsatellite loci. We propose the use of single-stranded conformation polymorphism (SSCP) analysis to identify unique clones prior to sequencing. We used this approach prior to sequencing from microsatellite-enriched libraries for three marine invertebrate species and were able to obtain a given number of unique clone sequences for only 28% of the sequencing effort that would have been required without SSCP screening.

Original languageEnglish
Pages (from-to)613-615
Number of pages3
JournalMolecular Ecology Notes
Volume6
Issue number3
DOIs
Publication statusPublished - Sep 2006

Keywords

  • Clones
  • Microsatellite enrichment
  • SSCP

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