Using a new fluorescent probe of silicification to measure species-specific activities of diatoms under varying environmental conditions

Bernard Queguiner; Karine Leblanc; Veronique Cornet-Barthaux; Leanne Armand; François Fripiat; D. Cardinal

doi:10.1007/978-90-481-8630-3_51

Using a new fluorescent probe of silicification to measure species-specific activities of diatoms under varying environmental conditions

Bernard Queguiner^*, Karine Leblanc, Veronique Cornet-Barthaux, Leanne Armand, François Fripiat, D. Cardinal

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution › peer-review

Abstract

A new method is presented that enables distinguishing between active and non-active cells with regard to biogenic silica deposition during frustule formation in natural communities of siliceous phytoplankton. The PDMPO method is based on the fluorescence of biogenic silica after incubation with the probe. Only those cells that have been depositing silica (by adjunction of intercalary plates during the cell cycle or by depositing a new frustule valve upon cell division) exhibit a typical fluorescence that is proportional to the amount of biogenic silica deposited. This new method has several advantages; it is easy to use at sea, very sensitive, and samples can be conserved for several months without major loss of fluorescence. This method offers new possibilities of investigation of ecophysiological controls within the natural diatom community and will also bring more information to the new generation of sophisticated multi-element multi-species biogeochemical models.

Original language	English
Title of host publication	Global change
Subtitle of host publication	mankind-marine environment interactions
Editors	Hubert-Jean Ceccaldi, Ivan Dekeyser, Mathias Girault, Georges Stora
Place of Publication	Dordrecht
Publisher	Springer, Springer Nature
Pages	283-287
Number of pages	5
ISBN (Electronic)	9789048186303
ISBN (Print)	9789048186297
DOIs	https://doi.org/10.1007/978-90-481-8630-3_51
Publication status	Published - 2011
Externally published	Yes
Event	13th French-Japanese Oceanography Symposium - Marseille, France Duration: 8 Sept 2008 → 12 Sept 2008

Conference

Conference	13th French-Japanese Oceanography Symposium
Country/Territory	France
City	Marseille
Period	8/09/08 → 12/09/08

Keywords

NORTHERN ADRIATIC SEA
FRESH-WATER INPUTS
P-SI RATIOS
BREST FRANCE
PHYTOPLANKTON COMMUNITIES
MACROTIDAL ECOSYSTEMS
BIOGENIC SILICA
DEPOSITION
BAY
IMPACTS

Access to Document

10.1007/978-90-481-8630-3_51

Cite this

Queguiner, B., Leblanc, K., Cornet-Barthaux, V., Armand, L., Fripiat, F., & Cardinal, D. (2011). Using a new fluorescent probe of silicification to measure species-specific activities of diatoms under varying environmental conditions. In H.-J. Ceccaldi, I. Dekeyser, M. Girault, & G. Stora (Eds.), Global change: mankind-marine environment interactions (pp. 283-287). Springer, Springer Nature. https://doi.org/10.1007/978-90-481-8630-3_51

Queguiner, Bernard ; Leblanc, Karine ; Cornet-Barthaux, Veronique et al. / Using a new fluorescent probe of silicification to measure species-specific activities of diatoms under varying environmental conditions. Global change: mankind-marine environment interactions. editor / Hubert-Jean Ceccaldi ; Ivan Dekeyser ; Mathias Girault ; Georges Stora. Dordrecht : Springer, Springer Nature, 2011. pp. 283-287

@inproceedings{7623a6bc96de4f8e87697013a75f9190,

title = "Using a new fluorescent probe of silicification to measure species-specific activities of diatoms under varying environmental conditions",

abstract = "A new method is presented that enables distinguishing between active and non-active cells with regard to biogenic silica deposition during frustule formation in natural communities of siliceous phytoplankton. The PDMPO method is based on the fluorescence of biogenic silica after incubation with the probe. Only those cells that have been depositing silica (by adjunction of intercalary plates during the cell cycle or by depositing a new frustule valve upon cell division) exhibit a typical fluorescence that is proportional to the amount of biogenic silica deposited. This new method has several advantages; it is easy to use at sea, very sensitive, and samples can be conserved for several months without major loss of fluorescence. This method offers new possibilities of investigation of ecophysiological controls within the natural diatom community and will also bring more information to the new generation of sophisticated multi-element multi-species biogeochemical models.",

keywords = "NORTHERN ADRIATIC SEA, FRESH-WATER INPUTS, P-SI RATIOS, BREST FRANCE, PHYTOPLANKTON COMMUNITIES, MACROTIDAL ECOSYSTEMS, BIOGENIC SILICA, DEPOSITION, BAY, IMPACTS",

author = "Bernard Queguiner and Karine Leblanc and Veronique Cornet-Barthaux and Leanne Armand and Fran{\c c}ois Fripiat and D. Cardinal",

year = "2011",

doi = "10.1007/978-90-481-8630-3_51",

language = "English",

isbn = "9789048186297",

pages = "283--287",

editor = "Hubert-Jean Ceccaldi and Ivan Dekeyser and Mathias Girault and Georges Stora",

booktitle = "Global change",

publisher = "Springer, Springer Nature",

address = "United States",

note = "13th French-Japanese Oceanography Symposium ; Conference date: 08-09-2008 Through 12-09-2008",

}

Queguiner, B, Leblanc, K, Cornet-Barthaux, V, Armand, L, Fripiat, F & Cardinal, D 2011, Using a new fluorescent probe of silicification to measure species-specific activities of diatoms under varying environmental conditions. in H-J Ceccaldi, I Dekeyser, M Girault & G Stora (eds), Global change: mankind-marine environment interactions. Springer, Springer Nature, Dordrecht, pp. 283-287, 13th French-Japanese Oceanography Symposium, Marseille, France, 8/09/08. https://doi.org/10.1007/978-90-481-8630-3_51

Using a new fluorescent probe of silicification to measure species-specific activities of diatoms under varying environmental conditions. / Queguiner, Bernard; Leblanc, Karine; Cornet-Barthaux, Veronique et al.
Global change: mankind-marine environment interactions. ed. / Hubert-Jean Ceccaldi; Ivan Dekeyser; Mathias Girault; Georges Stora. Dordrecht: Springer, Springer Nature, 2011. p. 283-287.

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding contribution › peer-review

TY - GEN

T1 - Using a new fluorescent probe of silicification to measure species-specific activities of diatoms under varying environmental conditions

AU - Queguiner, Bernard

AU - Leblanc, Karine

AU - Cornet-Barthaux, Veronique

AU - Armand, Leanne

AU - Fripiat, François

AU - Cardinal, D.

PY - 2011

Y1 - 2011

N2 - A new method is presented that enables distinguishing between active and non-active cells with regard to biogenic silica deposition during frustule formation in natural communities of siliceous phytoplankton. The PDMPO method is based on the fluorescence of biogenic silica after incubation with the probe. Only those cells that have been depositing silica (by adjunction of intercalary plates during the cell cycle or by depositing a new frustule valve upon cell division) exhibit a typical fluorescence that is proportional to the amount of biogenic silica deposited. This new method has several advantages; it is easy to use at sea, very sensitive, and samples can be conserved for several months without major loss of fluorescence. This method offers new possibilities of investigation of ecophysiological controls within the natural diatom community and will also bring more information to the new generation of sophisticated multi-element multi-species biogeochemical models.

AB - A new method is presented that enables distinguishing between active and non-active cells with regard to biogenic silica deposition during frustule formation in natural communities of siliceous phytoplankton. The PDMPO method is based on the fluorescence of biogenic silica after incubation with the probe. Only those cells that have been depositing silica (by adjunction of intercalary plates during the cell cycle or by depositing a new frustule valve upon cell division) exhibit a typical fluorescence that is proportional to the amount of biogenic silica deposited. This new method has several advantages; it is easy to use at sea, very sensitive, and samples can be conserved for several months without major loss of fluorescence. This method offers new possibilities of investigation of ecophysiological controls within the natural diatom community and will also bring more information to the new generation of sophisticated multi-element multi-species biogeochemical models.

KW - NORTHERN ADRIATIC SEA

KW - FRESH-WATER INPUTS

KW - P-SI RATIOS

KW - BREST FRANCE

KW - PHYTOPLANKTON COMMUNITIES

KW - MACROTIDAL ECOSYSTEMS

KW - BIOGENIC SILICA

KW - DEPOSITION

KW - BAY

KW - IMPACTS

U2 - 10.1007/978-90-481-8630-3_51

DO - 10.1007/978-90-481-8630-3_51

M3 - Conference proceeding contribution

SN - 9789048186297

SP - 283

EP - 287

BT - Global change

A2 - Ceccaldi, Hubert-Jean

A2 - Dekeyser, Ivan

A2 - Girault, Mathias

A2 - Stora, Georges

PB - Springer, Springer Nature

CY - Dordrecht

T2 - 13th French-Japanese Oceanography Symposium

Y2 - 8 September 2008 through 12 September 2008

ER -

Queguiner B, Leblanc K, Cornet-Barthaux V, Armand L, Fripiat F, Cardinal D. Using a new fluorescent probe of silicification to measure species-specific activities of diatoms under varying environmental conditions. In Ceccaldi HJ, Dekeyser I, Girault M, Stora G, editors, Global change: mankind-marine environment interactions. Dordrecht: Springer, Springer Nature. 2011. p. 283-287 doi: 10.1007/978-90-481-8630-3_51

Using a new fluorescent probe of silicification to measure species-specific activities of diatoms under varying environmental conditions

Abstract

Conference

Keywords

Access to Document

Fingerprint

Cite this