Using a new fluorescent probe of silicification to measure species-specific activities of diatoms under varying environmental conditions

Bernard Queguiner*, Karine Leblanc, Veronique Cornet-Barthaux, Leanne Armand, François Fripiat, D. Cardinal

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contributionpeer-review

Abstract

A new method is presented that enables distinguishing between active and non-active cells with regard to biogenic silica deposition during frustule formation in natural communities of siliceous phytoplankton. The PDMPO method is based on the fluorescence of biogenic silica after incubation with the probe. Only those cells that have been depositing silica (by adjunction of intercalary plates during the cell cycle or by depositing a new frustule valve upon cell division) exhibit a typical fluorescence that is proportional to the amount of biogenic silica deposited. This new method has several advantages; it is easy to use at sea, very sensitive, and samples can be conserved for several months without major loss of fluorescence. This method offers new possibilities of investigation of ecophysiological controls within the natural diatom community and will also bring more information to the new generation of sophisticated multi-element multi-species biogeochemical models.

Original languageEnglish
Title of host publicationGlobal change
Subtitle of host publicationmankind-marine environment interactions
EditorsHubert-Jean Ceccaldi, Ivan Dekeyser, Mathias Girault, Georges Stora
Place of PublicationDordrecht
PublisherSpringer, Springer Nature
Pages283-287
Number of pages5
ISBN (Electronic)9789048186303
ISBN (Print)9789048186297
DOIs
Publication statusPublished - 2011
Externally publishedYes
Event13th French-Japanese Oceanography Symposium - Marseille, France
Duration: 8 Sep 200812 Sep 2008

Conference

Conference13th French-Japanese Oceanography Symposium
CountryFrance
CityMarseille
Period8/09/0812/09/08

Keywords

  • NORTHERN ADRIATIC SEA
  • FRESH-WATER INPUTS
  • P-SI RATIOS
  • BREST FRANCE
  • PHYTOPLANKTON COMMUNITIES
  • MACROTIDAL ECOSYSTEMS
  • BIOGENIC SILICA
  • DEPOSITION
  • BAY
  • IMPACTS

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