Using meta-omics of contaminated sediments to monitor changes in pathways relevant to climate regulation

Simone C. Birrer*, Katherine A. Dafforn, Melanie Y. Sun, Rohan B. H. Williams, Jaimie Potts, Peter Scanes, Brendan P. Kelaher, Stuart L. Simpson, Staffan Kjelleberg, Sanjay Swarup, Peter Steinberg, Emma L. Johnston

*Corresponding author for this work

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    Microbially mediated biogeochemical processes are crucial for climate regulation and may be disrupted by anthropogenic contaminants. To better manage contaminants, we need tools that make real-time causal links between stressors and altered microbial functions, and the potential consequences for ecosystem services such as climate regulation. In a manipulative field experiment, we used metatranscriptomics to investigate the impact of excess organic enrichment and metal contamination on the gene expression of nitrogen and sulfur metabolisms in coastal sediments. Our gene expression data suggest that excess organic enrichment results in (i) higher transcript levels of genes involved in the production of toxic ammonia and hydrogen sulfide and (ii) lower transcript levels associated with the degradation of a greenhouse gas (nitrous oxide). However, metal contamination did not have any significant impact on gene expression. We reveal the genetic mechanisms that may lead to altered productivity and greenhouse gas production in coastal sediments due to anthropogenic contaminants. Our data highlight the applicability of metatranscriptomics as a management tool that provides an immense breadth of information and can identify potentially impacted process measurements that need further investigation.

    Original languageEnglish
    Pages (from-to)389-401
    Number of pages13
    JournalEnvironmental microbiology
    Volume21
    Issue number1
    Early online date8 Nov 2018
    DOIs
    Publication statusPublished - Jan 2019

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