Utilising glycobiology for fluorescent nanodiamond uptake and imaging in the central nervous system

Lindsay M. Parker, Philipp Reineck, Mina Ghanimi-Fard, Emma R. Wilson, Sameera Iqbal, Michael Baratta, Nicole M. Cordina, Anna Guller, Annemarie Nadort, Brant Gibson, Nicolle H. Packer

Research output: Chapter in Book/Report/Conference proceedingConference proceeding contribution

Abstract

Nanodiamonds are a carbon based class of nanoparticle quickly gaining popularity due to their low toxicity and versatile applications in biology and nanomedicine Their cheap and large scale synthesis, extensive optical characterisation and ease of bio-functionalisation also makes nanodiamonds an attractive material for use in bio-targeting studies Although central nervous system (CNS) cells are well known to functionally communicate via electrical and chemical signals, cell-surface glycans mediate the initial contact between cells and exogenous proteins. Glycosylation, the enzymatic process by which glycans are attached to proteins and lipids, is the most abundant and functionally important type of post-translational modification associated with brain development, neurodegenerative disorders, psychopathologies and brain cancers. The glycan structures on glycoproteins and glycolipids expressed in brain cells play key functional roles in neural development, biological processes and CNS maintenance such as cell adhesion, signal transduction, molecular trafficking and differentiation. Using EDC/NHS chemistry, we have coated fluorescent nanodiamonds with lectin proteins, which can recognise specific glycan receptor structures expressed on the cellular membrane of CNS cells There is substantial value in developing nanoparticle/lectin complexes for targeted nanoparticle based drug delivery in the CNS as their interaction with cell surface glycan receptors readily triggers endocytosis and subsequent trafficking to intracellular organelles such as endosomes and long term storage in the endoplasmic reticulum. We have engineered lectin coated fluorescent nanodiamonds and tested their ability to recognise specific CNS cell types in 2D and 3D models of brain cells in vitro and also applied them in vivo to rat brains Lectin coated nanodiamonds were successfully endocy-tosed by neurons, microglia and astrocytes in vitro and/or in vivo and remained in these cells for at least 48 hours with minimal stress to the host cells.

Original languageEnglish
Title of host publicationProceedings - 2019 PhotonIcs and Electromagnetics Research Symposium
Subtitle of host publicationPIERS 2019 Rome
Place of PublicationPiscataway, NJ
PublisherInstitute of Electrical and Electronics Engineers (IEEE)
Pages647-653
Number of pages7
ISBN (Electronic)9781728134031
DOIs
Publication statusPublished - 2019
Event2019 PhotonIcs and Electromagnetics Research Symposium - Spring, PIERS-Spring 2019 - Rome, Italy
Duration: 17 Jun 201920 Jun 2019

Publication series

NameProgress in Electromagnetics Research Symposium
Volume2019-June
ISSN (Print)1559-9450
ISSN (Electronic)1931-7360

Conference

Conference2019 PhotonIcs and Electromagnetics Research Symposium - Spring, PIERS-Spring 2019
CountryItaly
CityRome
Period17/06/1920/06/19

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Parker, L. M., Reineck, P., Ghanimi-Fard, M., Wilson, E. R., Iqbal, S., Baratta, M., ... Packer, N. H. (2019). Utilising glycobiology for fluorescent nanodiamond uptake and imaging in the central nervous system. In Proceedings - 2019 PhotonIcs and Electromagnetics Research Symposium: PIERS 2019 Rome (pp. 647-653). (Progress in Electromagnetics Research Symposium; Vol. 2019-June). Piscataway, NJ: Institute of Electrical and Electronics Engineers (IEEE). https://doi.org/10.1109/PIERS-Spring46901.2019.9017296